Food Engineering and Bioprocess Technology, Department of Food, Agriculture & Bioresources, Asian Institute of Technology, P.O. Box 4, Pathumthani 12120, Thailand.
Department of Veterinary Microbiology, Faculty of Animal Husbandry and Veterinary Sciences, Sindh Agriculture University, Tandojam 70060, Pakistan.
Oxid Med Cell Longev. 2023 Feb 7;2023:8475995. doi: 10.1155/2023/8475995. eCollection 2023.
The aim of this study was to investigate the antimicrobial and biopreservation potential of lactic acid bacteria. The potential probiotic culture inhibited the growth of gram-positive and gram-negative foodborne pathogens in agar spot assay with inhibition zones ranging from 10 to 21 mm in diameter. The strains showed coaggregation capabilities ranging from 7 to 71% with tested food pathogens including , , , and subsp. serovar Typhimurium. The effect of cell-free supernatants on the release of 260 nm absorbing material, especially nucleic acids, was evaluated and indicated the antagonistic activity on foodborne pathogens, the highest being against (3.77) and (3.86) after 60 min. The effect of cell-free supernatant (CFS) on the growth of pathogens showed that 11 and 93 had the highest inhibitory activity against tested strains. The biopreservation assay indicated that the potential probiotic strains 11 (BT), 19, 42, 60, 93, and 112 were effective in reducing the population in raw buffalo milk. Complete inhibition was observed after 6-8 days. This study showed that probiotic LAB from buffalo milk have antimicrobial and biopreservation potential; these strains have the potential to be utilized as biopreservative agents in food products.
本研究旨在探讨乳酸菌的抗菌和生物保鲜潜力。潜在的益生菌培养物在琼脂点分析中抑制了革兰氏阳性和革兰氏阴性食源性病原体的生长，抑菌圈直径为 10 至 21 毫米。这些菌株与测试的食源性病原体（包括 、 、 和 血清型 Typhimurium）具有 7 至 71%的共聚能力。评估了无细胞上清液对 260nm 吸光物质（尤其是核酸）释放的影响，表明其对食源性病原体具有拮抗活性，对 （3.77）和 （3.86）的最高抑制活性分别为 （60 分钟后）和 （60 分钟后）。无细胞上清液（CFS）对病原体生长的影响表明，11 和 93 对测试菌株的抑制活性最高。生物保鲜试验表明，潜在的益生菌菌株 11（BT）、19、42、60、93 和 112 可有效降低生水牛乳中的 菌数。在 6-8 天后可观察到完全抑制。本研究表明，水牛乳源益生菌 LAB 具有抗菌和生物保鲜潜力；这些菌株有潜力作为食品中的生物保鲜剂。
