Sharma Anjali, Lavania Meeta, Singh Raghvendar, Lal Banwari
Microbial Biotechnology, Environmental and Industrial Biotechnology Division, The Energy and Resources Institute (TERI), New Delhi, India.
Department of Biochemistry, National Research Centre on Camel, Jorbeer, Bikaner, India.
Saudi J Biol Sci. 2021 Mar;28(3):1622-1632. doi: 10.1016/j.sjbs.2020.11.062. Epub 2020 Nov 24.
In the present study, a total of 80 presumed lactic acid bacteria (LAB) were isolated from camel milk. Selected LAB were identified as (cam 12), (cam 14) and (cam 15) and their potential were tested by tolerance & de-conjugation of bile salts, antimicrobial activity, surface hydrophobicity and adhesion potential) along with this of probiotics were evaluated for curd formation and assessed for sensory properties and syneresis. Selected LABs showed antimicrobial activity against wide range of pathogenic bacteria ( and . LAB (cam 12, cam 14 and cam15) were highly sceptible to chloramphenicol, vancomycin, and tetracyclin. In vitro adhesion studies with Caco-2 cells demonstrated strong adhesion activity with hydrophobicity (99%) was observed. Acute oral toxicity of . and . showed non-toxic, non-virulent and safe for industrial application. The study provides potential LAB which may act as a substitute of functional food, synthetic feed and industrial curd formulation with in the shortest span (240 min at 28-32 °C).
在本研究中，共从骆驼奶中分离出80株疑似乳酸菌（LAB）。选定的LAB被鉴定为（cam 12）、（cam 14）和（cam 15），并通过胆汁盐的耐受性和去结合、抗菌活性、表面疏水性和黏附潜力对其潜力进行了测试，同时评估了益生菌在凝乳形成方面的表现，并对其感官特性和脱水收缩进行了评估。选定的LAB对多种病原菌（和）具有抗菌活性。LAB（cam 12、cam 14和cam15）对氯霉素、万古霉素和四环素高度敏感。与Caco-2细胞的体外黏附研究表明其具有较强的黏附活性，观察到疏水性为99%。和的急性经口毒性表明其无毒、无致病性且对工业应用安全。该研究提供了具有潜力的LAB，其可在最短时间内（28 - 32°C下240分钟）替代功能性食品、合成饲料和工业凝乳配方。
