Ralston Tyler S, Marks Daniel L, Carney P Scott, Boppart Stephen A
Beckman Institute for Advanced Science and Technology, University of Illinois at Urbana-Champaign, 405 N. Mathews Avenue, Urbana, Illinois 61801, USA ; Department of Electrical and Computer Engineering, University of Illinois at Urbana-Champaign, 405 N. Mathews Avenue, Urbana, Illinois 61801, USA.
Beckman Institute for Advanced Science and Technology, University of Illinois at Urbana-Champaign, 405 N. Mathews Avenue, Urbana, Illinois 61801, USA ; Department of Electrical and Computer Engineering, University of Illinois at Urbana-Champaign, 405 N. Mathews Avenue, Urbana, Illinois 61801, USA ; Departments of Bioengineering, Internal Medicine, University of Illinois at Urbana-Champaign, 405 N. Mathews Avenue, Urbana, Illinois 61801, USA.
Nat Phys. 2007 Feb 1;3(2):129-134. doi: 10.1038/nphys514.
State-of-the-art methods in high-resolution three-dimensional optical microscopy require that the focus be scanned through the entire region of interest. However, an analysis of the physics of the light-sample interaction reveals that the Fourier-space coverage is independent of depth. Here we show that, by solving the inverse scattering problem for interference microscopy, computed reconstruction yields volumes with a resolution in all planes that is equivalent to the resolution achieved only at the focal plane for conventional high-resolution microscopy. In short, the entire illuminated volume has spatially invariant resolution, thus eliminating the compromise between resolution and depth of field. We describe and demonstrate a novel computational image-formation technique called interferometric synthetic aperture microscopy (ISAM). ISAM has the potential to broadly impact real-time three-dimensional microscopy and analysis in the fields of cell and tumour biology, as well as in clinical diagnosis where imaging is preferable to biopsy.
高分辨率三维光学显微镜的先进方法要求焦点扫描整个感兴趣区域。然而，对光与样品相互作用的物理分析表明，傅里叶空间覆盖范围与深度无关。在此我们表明，通过解决干涉显微镜的逆散射问题，计算重建得到的体积在所有平面上的分辨率都等同于传统高分辨率显微镜仅在焦平面上实现的分辨率。简而言之，整个照明体积具有空间不变的分辨率，从而消除了分辨率与景深之间的折衷。我们描述并展示了一种名为干涉合成孔径显微镜（ISAM）的新型计算图像形成技术。ISAM有潜力广泛影响细胞和肿瘤生物学领域以及临床诊断（在临床诊断中成像比活检更可取）中的实时三维显微镜检查和分析。
