Liu Yuan-Zhi, Shemonski Nathan D, Adie Steven G, Ahmad Adeel, Bower Andrew J, Carney P Scott, Boppart Stephen A
Beckman Institute for Advanced Science and Technology, University of Illinois at Urbana-Champaign, 405 North Mathews Avenue, Urbana, Illinois 61801, USA ; Department of Electrical and Computer Engineering, University of Illinois at Urbana-Champaign, 1406 West Green Street, Urbana, Illinois 61801, USA.
Department of Biomedical Engineering, Cornell University, 101 Weill Hall, Ithaca, New York 14853, USA.
Biomed Opt Express. 2014 Aug 8;5(9):2988-3000. doi: 10.1364/BOE.5.002988. eCollection 2014 Sep 1.
Three-dimensional high-resolution imaging methods are important for cellular-level research. Optical coherence microscopy (OCM) is a low-coherence-based interferometry technology for cellular imaging with both high axial and lateral resolution. Using a high-numerical-aperture objective, OCM normally has a shallow depth of field and requires scanning the focus through the entire region of interest to perform volumetric imaging. With a higher-numerical-aperture objective, the image quality of OCM is affected by and more sensitive to aberrations. Interferometric synthetic aperture microscopy (ISAM) and computational adaptive optics (CAO) are computed imaging techniques that overcome the depth-of-field limitation and the effect of optical aberrations in optical coherence tomography (OCT), respectively. In this work we combine OCM with ISAM and CAO to achieve high-speed volumetric cellular imaging. Experimental imaging results of ex vivo human breast tissue, ex vivo mouse brain tissue, in vitro fibroblast cells in 3D scaffolds, and in vivo human skin demonstrate the significant potential of this technique for high-speed volumetric cellular imaging.
三维高分辨率成像方法对于细胞水平的研究至关重要。光学相干显微镜（OCM）是一种基于低相干干涉测量技术的细胞成像方法，具有高轴向分辨率和横向分辨率。使用高数值孔径物镜时，OCM通常景深较浅，需要在整个感兴趣区域扫描焦点以进行体积成像。数值孔径越高，OCM的图像质量受像差影响越大且对其更敏感。干涉合成孔径显微镜（ISAM）和计算自适应光学（CAO）分别是克服光学相干断层扫描（OCT）中景深限制和光学像差影响的计算成像技术。在这项工作中，我们将OCM与ISAM和CAO相结合，以实现高速体积细胞成像。对离体人乳腺组织、离体小鼠脑组织、三维支架中的体外成纤维细胞以及体内人体皮肤的实验成像结果证明了该技术在高速体积细胞成像方面的巨大潜力。
