Assaad Houssein I, Hou Yongqing, Zhou Lan, Carroll Raymond J, Wu Guoyao
Department of Statistics, Texas A & M University, College Station, TX 77843 USA ; Department of Animal Science, Texas A & M University, College Station, TX 77843 USA.
Hubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University, Wuhan, 430023 China.
Springerplus. 2015 Jan 23;4:33. doi: 10.1186/s40064-015-0795-z. eCollection 2015.
Statistical tables are an essential component of scientific papers and reports in biomedical and agricultural sciences. Measurements in these tables are summarized as mean ± SEM for each treatment group. Results from pairwise-comparison tests are often included using letter displays, in which treatment means that are not significantly different, are followed by a common letter. However, the traditional manual processes for computation and presentation of statistically significant outcomes in MS Word tables using a letter-based algorithm are tedious and prone to errors.
Using the R package 'Shiny', we present a web-based program freely available online, at https://houssein-assaad.shinyapps.io/TwoWayANOVA/. No download is required. The program is capable of rapidly generating publication-ready tables containing two-way analysis of variance (ANOVA) results. Additionally, the software can perform multiple comparisons of means using the Duncan, Student-Newman-Keuls, Tukey Kramer, Westfall, and Fisher's least significant difference (LSD) tests. If the LSD test is selected, multiple methods (e.g., Bonferroni and Holm) are available for adjusting p-values. Significance statements resulting from all pairwise comparisons are included in the table using the popular letter display algorithm. With the application of our software, the procedures of ANOVA can be completed within seconds using a web-browser, preferably Mozilla Firefox or Google Chrome, and a few mouse clicks. To our awareness, none of the currently available commercial (e.g., Stata, SPSS and SAS) or open-source software (e.g., R and Python) can perform such a rapid task without advanced knowledge of the corresponding programming language.
The new and user-friendly program described in this paper should help scientists perform statistical analysis and rapidly generate publication-ready MS-Word tables for two-way ANOVA. Our software is expected to facilitate research in agriculture, biomedicine, and other fields of life sciences.
统计表是生物医学和农业科学领域科学论文及报告的重要组成部分。这些表格中的测量数据以每个处理组的均值±标准误进行汇总。成对比较检验的结果通常采用字母标注法呈现，即均值无显著差异的处理组后面跟着一个共同的字母。然而，在Microsoft Word表格中使用基于字母的算法来计算和呈现具有统计学意义的结果的传统手动流程既繁琐又容易出错。
我们使用R包“Shiny”开发了一个基于网络的程序，可在https://houssein - assaad.shinyapps.io/TwoWayANOVA/免费在线获取，无需下载。该程序能够快速生成包含双向方差分析（ANOVA）结果且可供发表的表格。此外，该软件可以使用邓肯检验、学生 - 纽曼 - 库尔斯检验、图基 - 克雷默检验、韦斯特福尔检验和费舍尔最小显著差异（LSD）检验进行均值的多重比较。如果选择LSD检验，则有多种方法（如邦费罗尼法和霍尔姆法）可用于调整p值。使用流行的字母标注算法，所有成对比较得出的显著性声明都会包含在表格中。通过应用我们的软件，使用网络浏览器（最好是Mozilla Firefox或Google Chrome）并点击几下鼠标，ANOVA的程序就能在几秒钟内完成。据我们所知，目前可用的商业软件（如Stata、SPSS和SAS）或开源软件（如R和Python），如果没有相应编程语言的高级知识，都无法如此快速地完成这项任务。
本文所描述的这个全新且用户友好的程序应有助于科学家进行统计分析，并快速生成可供发表的双向ANOVA的Microsoft Word表格。我们的软件有望促进农业、生物医学和生命科学其他领域的研究。
