Ganesh P Sankar, Vittal Ravishankar Rai
a Department of Studies in Microbiology , University of Mysore , Manasagangotri, Mysore 570006 , Karnataka , India.
Nat Prod Res. 2015;29(24):2295-8. doi: 10.1080/14786419.2015.1004673. Epub 2015 Jan 30.
The antibiofilm activity of Murraya koenigii essential oil (EO) against Pseudomonas aeruginosa PAO1 was investigated in this study. A decrease in the production of rhamnolipid, extracellular polymeric substance and swarming motility was observed by the EO treatment (0.3% v/v). The static microtitre plate assay revealed 80% reduction in biofilm formation by P. aeruginosa PAO1 on M. koenigii EO treatment. Fluorescence microscopy and scanning electron microscopy analyses confirmed the reduction of biofilm formation in P. aeruginosa PAO1 when treated with M. koenigii EO. Gas chromatography-mass spectrometry analysis of the EO revealed the presence of well-known antibiofilm agents such as spathulenol (5.85%), cinnamaldehyde (0.37%) and linalool (0.04%). Cinnamaldehyde has not been previously reported in M. koenigii EO. The potent antibiofilm properties of M. koenigii EO may be effectively exploited in food and pharmaceutical industries as well as in controlling Pseudomonas biofilms on indwelling medical devices.
本研究考察了九里香精油（EO）对铜绿假单胞菌PAO1的抗生物膜活性。通过EO处理（0.3% v/v）观察到鼠李糖脂、胞外聚合物的产生以及群体运动性均有所降低。静态微量滴定板试验显示，经九里香EO处理后，铜绿假单胞菌PAO1的生物膜形成减少了80%。荧光显微镜和扫描电子显微镜分析证实，用九里香EO处理后，铜绿假单胞菌PAO1的生物膜形成减少。对该EO的气相色谱 - 质谱分析表明，存在诸如匙叶桉油烯醇（5.85%）、肉桂醛（0.37%）和芳樟醇（0.04%）等知名抗生物膜剂。此前尚未在九里香EO中报道过肉桂醛。九里香EO强大的抗生物膜特性可在食品和制药行业以及控制植入式医疗器械上的假单胞菌生物膜方面得到有效利用。
