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评估精油作为潜在根管冲洗剂的抗菌和抗生物膜特性的体外方法——技术步骤的简要描述

In Vitro Methods for Assessing the Antibacterial and Antibiofilm Properties of Essential Oils as Potential Root Canal Irrigants-A Simplified Description of the Technical Steps.

作者信息

Diouchi Jihad, Marinković Jelena, Nemoda Milica, El Rhaffari Lhoussaine, Toure Babacar, Ghoul Sonia

机构信息

Health Sciences Research Center, International Faculty of Dental Medicine, College of Health Sciences, International University of Rabat, Technopolis Parc, Rocade of Rabat-Salé, Sala-Al Jadida 11100, Morocco.

National Institute of the Republic of Serbia, 'VINCA' Institute of Nuclear Sciences, University of Belgrade, Mike Petrovica Alasa 12, 11000 Belgrade, Serbia.

出版信息

Methods Protoc. 2024 Jun 25;7(4):50. doi: 10.3390/mps7040050.

DOI:10.3390/mps7040050
PMID:39051264
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11270344/
Abstract

BACKGROUND

Essential oils have gained in significance due to their various biological activities, and there is a growing demand for them in many industries. The present article focuses on the technical steps for an in vitro evaluation of the antibacterial and antibiofilm activities of essential oils for potential use as root canal irrigant in dentistry.

METHODS

The bioactivities of the essential oil were investigated through in vitro assays. The gram-positive bacterium was used as a micro-organism model. The antibacterial activity of the essential oil was assessed using the microdilution method, and resazurin staining to determine the minimal inhibition concentrations (MICs) and the minimal bactericidal concentrations (MBCs). The antibiofilm effect was evaluated spectrophotometrically at 570 nm using the microplate cultivation technique and crystal violet staining.

CONCLUSIONS

This article features a detailed in vitro protocol to facilitate the preparation of the essential oil samples, the bacterial suspension, and the methods used for assessment of the antibiofilm and antibacterial activities of the essential oil. The advantages of these approaches are presented in relation to the limits linked to the choice of the bacteria and the essential oil.

摘要

背景

由于其多种生物活性，精油的重要性日益凸显，许多行业对其需求不断增长。本文重点介绍了体外评估精油抗菌和抗生物膜活性的技术步骤，这些精油有可能用作牙科根管冲洗剂。

方法

通过体外试验研究精油的生物活性。使用革兰氏阳性菌作为微生物模型。采用微量稀释法评估精油的抗菌活性，并使用刃天青染色法确定最低抑菌浓度（MIC）和最低杀菌浓度（MBC）。使用微孔板培养技术和结晶紫染色，在570nm处通过分光光度法评估抗生物膜效果。

结论

本文介绍了一个详细的体外实验方案，以方便制备精油样品、细菌悬液，以及用于评估精油抗生物膜和抗菌活性的方法。相对于与细菌和精油选择相关的局限性，还介绍了这些方法的优点。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bbc1/11270344/940b4b603b97/mps-07-00050-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bbc1/11270344/c96b7fd0e05b/mps-07-00050-g001.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bbc1/11270344/dee55ac2662e/mps-07-00050-g003.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bbc1/11270344/940b4b603b97/mps-07-00050-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bbc1/11270344/c96b7fd0e05b/mps-07-00050-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bbc1/11270344/18eb928f9e9b/mps-07-00050-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bbc1/11270344/dee55ac2662e/mps-07-00050-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bbc1/11270344/b143545395ab/mps-07-00050-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bbc1/11270344/940b4b603b97/mps-07-00050-g005.jpg

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Pharmacy (Basel). 2023 Feb 10;11(1):33. doi: 10.3390/pharmacy11010033.
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Applications of Essential Oils and Plant Extracts in Different Industries.精油和植物提取物在不同行业的应用。
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Prevalence of root canal treatment worldwide: A systematic review and meta-analysis.全球范围内根管治疗的流行率：系统评价和荟萃分析。
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