Department of Mechanical Engineering, Korea University, Seoul 136-701, Republic of Korea.
Department of Chemical and Biomolecular Engineering, Yonsei University, Seoul 120-749, Republic of Korea.
Biosens Bioelectron. 2015 Jun 15;68:481-486. doi: 10.1016/j.bios.2015.01.036. Epub 2015 Jan 19.
Zinc oxide nanowires (ZnO NWs) have been attempted to various applications, such as piezoelectric devices, energy harvesting devices, self-powered nanosensors, and biomedical devices. However, recent reports have shown the toxic effect of ZnO NWs. In this report, we described the detection of ZnO NWs, for the first time using reduced graphene oxide (RGO) wrapping method. By wrapping RGO to ZnO NW (RGO-ZnO NW), we are able to aggregate ZnO NWs and increase the sensing performance. The detection measurement is based on the resonance frequency shift derived from mass variation of RGO-ZnO NW adsorption on the DNA immobilized resonator. The resonator is able to detect ZnO NWs with detection limit of 100 ng mL(-1) which is 2 order below the fatal toxic concentration of ZnO NWs in Human Monocyte Macrophages (HMMs). Furthermore, the resonator is able to detect ZnO NWs in real tap water, showing the potential as ZnO NWs screening platform in real environmental aqua system.
氧化锌纳米线（ZnO NWs）已被尝试应用于各种领域，如压电设备、能量收集设备、自供电纳米传感器和生物医学设备。然而，最近的报告显示 ZnO NWs 具有毒性效应。在本报告中，我们首次描述了使用还原氧化石墨烯（RGO）包裹法检测 ZnO NWs。通过将 RGO 包裹到 ZnO NW（RGO-ZnO NW）上，我们能够聚集 ZnO NWs 并提高传感性能。检测测量基于共振频率的变化，共振频率的变化源于 RGO-ZnO NW 在固定于 DNA 的谐振器上吸附引起的质量变化。该谐振器能够检测到 ZnO NWs，检测限为 100ng/mL，这比 ZnO NWs 在人单核巨噬细胞（HMMs）中的致命毒性浓度低 2 个数量级。此外，该谐振器还能够检测到实际自来水中的 ZnO NWs，显示了其作为实际环境水系统中 ZnO NWs 筛选平台的潜力。
