WATLab and Department of Chemistry, University of Waterloo, Waterloo, Ontario, Canada N2L 3G1.
ACS Appl Mater Interfaces. 2010 Aug;2(8):2409-12. doi: 10.1021/am100413u.
The present work demonstrates the fabrication and performance of an enzymatic glucose biosensor based on ZnO nanowires (NWs) deposited on a Au-coated polyester (PET) substrate. Electrodeposition of ZnO NWs on the conducting PET substrate was carried out at 70 degrees C in an aqueous electrolyte consisting of zinc nitrate mixed with potassium chloride. Glucose oxidase (GOx) was subsequently immobilized on the as-synthesized ZnO NWs, and the electrocatalytic properties of GOx-immobilized ZnO NWs were evaluated by amperometry. The resulting GOx/ZnO-NWs/Au/PET bioelectrode exhibits excellent electrocatalytic performance with a high sensitivity of 19.5 muA mM(-1) cm(-2), a low Michaelis-Menten constant of 1.57 mM, and a fast response time of <5 s for the amperometric detection of glucose. The present study illustrates the feasibility of realizing light-weight, flexible, high-performance sensing devices using ZnO NWs.
本工作展示了基于在镀金聚酯（PET）基底上沉积的氧化锌纳米线（NWs）的酶葡萄糖生物传感器的制造和性能。在包含硝酸锌和氯化钾的水溶液电解质中，在 70°C 下进行导电 PET 基底上的 ZnO NWs 的电沉积。随后将葡萄糖氧化酶（GOx）固定在合成的 ZnO NWs 上，并通过安培法评估 GOx 固定化 ZnO NWs 的电催化性能。所得的 GOx/ZnO-NWs/Au/PET 生物电极具有出色的电催化性能，对葡萄糖的安培检测表现出 19.5 μA mM(-1) cm(-2)的高灵敏度、1.57 mM 的低米氏常数和<5 s 的快速响应时间。本研究说明了使用 ZnO NWs 实现轻量级、灵活、高性能传感设备的可行性。
