Sartore G, Seraglia R, Burlina S, Bolis A, Marin R, Manzato E, Ragazzi E, Traldi P, Lapolla A
Department of Medicine - DIMED, University of Padova, Italy.
CNR-IENI, Padova, Italy.
Nutr Metab Cardiovasc Dis. 2015 Apr;25(4):418-25. doi: 10.1016/j.numecd.2014.12.004. Epub 2014 Dec 19.
ApoA-I can undergo oxidative changes that reduce anti-atherogenic role of HDL. The aim of this study was to seek any significant differences in methionine sulfoxide (MetO) content in the ApoA-I of HDL isolated from young patients with coronary heart disease (CHD), type 2 diabetics and healthy subjects.
We evaluated the lipid profile of 21 type 2 diabetic patients, 23 young patients with premature MI and 21 healthy volunteers; we determined in all patients the MetO content of ApoA-I in by MALDI/TOF/TOF technique. The typical MALDI spectra of the tryptic digest obtained from HDL plasma fractions all patients showed a relative abundance of peptides containing Met(112)O in ApoA-I in type 2 diabetic and CHD patients. This relative abundance is given as percentages of oxidized ApoA-I (OxApoA-I). OxApoA-I showed no significant correlations with lipoproteins in all patients studied, while a strong correlation emerged between the duration of diabetic disease and OxApoA-I levels in type 2 diabetic patients.
The most remarkable finding of our study lies in the evidence it produced of an increased HDL oxidation in patients highly susceptible to CHD. Levels of MetO residues in plasma ApoA-I, measured using an accurate, specific method, should be investigated and considered in prospective future studies to assess their role in CHD.
载脂蛋白A-I（ApoA-I）可发生氧化变化，从而降低高密度脂蛋白（HDL）的抗动脉粥样硬化作用。本研究旨在探寻从年轻冠心病（CHD）患者、2型糖尿病患者及健康受试者中分离出的HDL的ApoA-I中蛋氨酸亚砜（MetO）含量是否存在显著差异。
我们评估了21例2型糖尿病患者、23例年轻早发心肌梗死患者和21例健康志愿者的血脂谱；通过基质辅助激光解吸电离飞行时间串联质谱（MALDI/TOF/TOF）技术测定了所有患者ApoA-I中的MetO含量。所有患者HDL血浆组分胰蛋白酶消化产物的典型MALDI光谱显示，2型糖尿病患者和CHD患者的ApoA-I中含Met(112)O的肽段相对丰度较高。这种相对丰度以氧化型ApoA-I（OxApoA-I）的百分比表示。在所有研究患者中，OxApoA-I与脂蛋白均无显著相关性，而在2型糖尿病患者中，糖尿病病程与OxApoA-I水平之间存在强烈相关性。
我们研究最显著的发现在于，它证明了易患CHD的患者中HDL氧化增加。在未来前瞻性研究中，应采用准确、特异的方法检测并考虑血浆ApoA-I中MetO残基的水平，以评估其在CHD中的作用。
