Division of Biophysics and Bioimaging, Ontario Cancer Institute and Department of Medical Biophysics, University of Toronto, 610 University Avenue, Toronto, ON M5G 2M9, Canada.
Phys Med Biol. 2011 Apr 21;56(8):N115-22. doi: 10.1088/0031-9155/56/8/N01. Epub 2011 Mar 28.
Formalin fixation is a preparation method widely used in handling tissue specimens, such as biopsies, specifically in optical studies such as microscopy. In this note, we examine how formalin fixation affects the polarization properties of porcine myocardium and liver as assessed by optical polarimetry. Spatial maps of linear retardance and depolarization were derived from four myocardial and four liver samples before and after formalin fixation. Overall, linear retardance and depolarization increased after fixation for both myocardium (15% and 23% increase, respectively) and liver (38% and 51%, respectively). The relative increase in retardance was greater in liver compared to myocardium, although the absolute increase in retardance was comparable for both. The effect of fixation on bulk optical properties was also investigated for myocardium where the scattering coefficient increased from 92 to 132 cm(-1) and the absorption coefficient remained constant at 1.1 cm(-1).
福尔马林固定是一种广泛应用于处理组织标本（如活检）的制备方法，特别是在光学研究如显微镜检查中。在本说明中，我们研究了福尔马林固定如何影响光学偏振测量评估的猪心肌和肝脏的偏振特性。在福尔马林固定前后，从四个心肌和四个肝脏样本中得出了线性延迟和去偏振的空间图谱。总体而言，固定后心肌（分别增加 15%和 23%）和肝脏（分别增加 38%和 51%）的线性延迟和去偏振均增加。与心肌相比，肝脏的延迟相对增加更大，尽管两者的延迟绝对值相当。还研究了固定对心肌的体光学性质的影响，其中散射系数从 92cm(-1)增加到 132cm(-1)，而吸收系数在 1.1cm(-1)处保持不变。
