Brzezinska Elspeth A, Nixon Colin, Patel Rachana, Leung Hing Y
The Beatson Institute for Cancer Research, Garscube Estate, Switchback Road, G61 1BD, Bearsden, Glasgow, UK.
Methods Mol Biol. 2015;1267:73-91. doi: 10.1007/978-1-4939-2297-0_4.
Genetically engineered mouse models have become fundamental tools in the basic and translational research of prostate cancer. There is a plethora of models available to dissect the genetic alterations and aberrant signaling events associated with human prostate cancer and, furthermore, to investigate new and "personalized" therapies to treat the disease. In this chapter, we discuss some of the models recently and currently used to study prostate cancer in vivo, and some considerations when selecting an appropriate model to investigate particular aspects of the disease. We describe the methods required to isolate prostate tumors and conduct basic characterization of the tumor to determine tumor load and histopathology. We also discuss important aspects to be considered when processing samples for further analysis.
基因工程小鼠模型已成为前列腺癌基础研究和转化研究的基本工具。有大量模型可用于剖析与人类前列腺癌相关的基因改变和异常信号事件，此外，还可用于研究治疗该疾病的新的“个性化”疗法。在本章中，我们将讨论最近和目前用于体内研究前列腺癌的一些模型，以及选择合适模型来研究该疾病特定方面时的一些注意事项。我们描述了分离前列腺肿瘤并对肿瘤进行基本特征分析以确定肿瘤负荷和组织病理学所需的方法。我们还讨论了在处理样本以进行进一步分析时需要考虑的重要方面。
