Laboratory of cardiovascular Physiology and physical activity, Institute of Bioscience, Univ Estadual Paulista (UNESP), Rio Claro, SP, Brazil.
Department of Structural and Functional Biology, Institute of Biology, University of Campinas, Campinas, SP, Brazil.
Life Sci. 2015 Mar 15;125:63-70. doi: 10.1016/j.lfs.2014.12.021. Epub 2015 Jan 28.
To examine the influence of l-arginine supplementation in combination with physical training on mitochondrial biomarkers from gastrocnemius muscle and its relationship with physical performance.
Male Wistar rats were divided into four groups: control sedentary (SD), sedentary supplemented with l-arginine (SDLA), trained (TR) and trained supplemented with l-arginine (TRLA). Supplementation of l-arginine was administered by gavage (62.5mg/ml/day/rat). Physical training consisted of 60min/day, 5days/week, 0% grade, speed of 1.2km/h. The study lasted 8weeks. Skeletal muscle mitochondrial enriched fraction as well as cytoplasmic fractions were obtained for Western blotting and biochemical analyses. Protein expressions of transcriptor coactivator (PGC-1α), transcriptor factors (mtTFA), ATP synthase subunit c, cytochrome oxidase (COXIV), constitutive nitric oxide synthases (eNOS and nNOS), Cu/Zn-superoxide dismutase (SOD) and manganese-SOD (Mn-SOD) were evaluated. We also assessed in plasma: lipid profile, glycemia and malondialdehyde (MDA) levels. The nitrite/nitrate (NOx(-)) levels were measured in both plasma and cytosol fraction of the gastrocnemius muscle.
8-week l-arginine supplementation associated with physical training was effective in promoting greater tolerance to exercise that was accompanied by up-regulation of the protein expressions of mtTFA, PGC-1α, ATP synthase subunit c, COXIV, Cu/Zn-SOD and Mn-SOD. The upstream pathway was associated with improvement of NO bioavailability, but not in NO production since no changes in nNOS or eNOS protein expressions were observed.
This combination would be an alternative approach for preventing cardiometabolic diseases given that in overt diseases a profound impairment in the physical performance of the patients is observed.
研究精氨酸补充联合体育锻炼对腓肠肌线粒体生物标志物的影响及其与运动表现的关系。
雄性 Wistar 大鼠分为 4 组：安静对照组（SD）、安静补充精氨酸组（SDLA）、运动组（TR）和运动补充精氨酸组（TRLA）。精氨酸补充通过灌胃（62.5mg/ml/天/只）进行。体育锻炼包括每天 60 分钟，每周 5 天，坡度为 0%，速度为 1.2km/h。研究持续 8 周。获得骨骼肌线粒体富集部分和细胞质部分，用于 Western blot 和生化分析。评估转录共激活因子（PGC-1α）、转录因子（mtTFA）、ATP 合酶亚基 c、细胞色素氧化酶（COXIV）、组成型一氧化氮合酶（eNOS 和 nNOS）、铜/锌-超氧化物歧化酶（Cu/Zn-SOD）和锰-SOD（Mn-SOD）的蛋白表达。还检测了血浆中的脂质谱、血糖和丙二醛（MDA）水平。测量了血浆和腓肠肌细胞质部分的亚硝酸盐/硝酸盐（NOx(-)）水平。
8 周的精氨酸补充联合体育锻炼能有效提高运动耐受力，同时上调 mtTFA、PGC-1α、ATP 合酶亚基 c、COXIV、Cu/Zn-SOD 和 Mn-SOD 的蛋白表达。上游途径与改善 NO 生物利用度有关，但与 NO 产生无关，因为 nNOS 或 eNOS 的蛋白表达没有变化。
鉴于在明显的疾病中，患者的运动表现会受到严重损害，因此这种联合用药可能是预防心脏代谢疾病的一种替代方法。
