Laurindo Caroline Pancera, Rego Gregorio Karen C, Rippi Moreno Ana Caroline, Viudes Agostinho Julia Maia, Campos Evelyn Carvalho, Nai Gisele Alborghetti, Nunes Maria Tereza, Seraphim Patrícia Monteiro
Department of Physiotherapy - School of Sciences and Technology - Sao Paulo, State University - UNESP, Campus Presidente Prudente, Brazil.
Department of Physiology and Biophysics - Institute of Biomedical Sciences I, University of Sao Paulo, Sao Paulo, SP, Brazil.
Heliyon. 2021 Nov 14;7(11):e08374. doi: 10.1016/j.heliyon.2021.e08374. eCollection 2021 Nov.
To investigate the effect of resistance training (RT) on hepatocardiovascular and muscle mitochondrial parameters in rats that were fed a high-calorie diet for 12 weeks.
The animals were divided into four groups: control (C), exercise (E), obese (O), and obese plus exercise (OE). Group E and OE rats performed resistance training by climbing on a vertical ladder with load attached to the end of the tail (1×/day, 3×/week, for 12 weeks). Group O and OE rats were fed a high-calorie diet containing chow and a cafeteria diet for 12 weeks. Under anesthesia, the heart and liver were removed for histopathological analysis, and the gastrocnemius muscle was removed for Western blotting.
Group O rats were heavier, with increased fat mass, elevated fasting glycemia, and total triglycerides, and exhibited a significant number of Kupffer cells and diffuse steatosis in the liver. Group O rats also showed increased thickness of the right ventricle, septum, and pulmonary artery. All of these parameters were attenuated by RT. PGC1-α protein levels were increased in both exercise groups. The protein levels of OXPHOS complexes III, IV, and V were reduced in Group O, while RT prevented this alteration.
RT exerts a protective effect against hepato-cardiac alterations and prevents changes in the muscle mitochondrial protein profile induced by a high-calorie diet.
研究抗阻训练（RT）对喂食高热量饮食12周的大鼠肝脏、心脏和肌肉线粒体参数的影响。
将动物分为四组：对照组（C）、运动组（E）、肥胖组（O）和肥胖加运动组（OE）。E组和OE组大鼠通过攀爬末端系有重物的垂直梯子进行抗阻训练（每天1次，每周3次，共12周）。O组和OE组大鼠喂食含普通饲料和自选食物的高热量饮食12周。在麻醉状态下，取出心脏和肝脏进行组织病理学分析，取出腓肠肌进行蛋白质免疫印迹分析。
O组大鼠体重更重，脂肪量增加，空腹血糖和总甘油三酯升高，肝脏中库普弗细胞数量显著增加且出现弥漫性脂肪变性。O组大鼠还表现出右心室、室间隔和肺动脉厚度增加。所有这些参数通过抗阻训练均有所减轻。两个运动组中PGC1-α蛋白水平均升高。O组中氧化磷酸化复合体III、IV和V的蛋白水平降低，而抗阻训练可防止这种改变。
抗阻训练对肝脏和心脏改变具有保护作用，并可防止高热量饮食引起的肌肉线粒体蛋白质谱变化。
