Gwak Joo-Han, Kim So-Jeong, Jung Man-Young, Kim Jong-Geol, Roh Seong Woon, Yim Kyung June, Lee Yong-Jae, Kim Song-Gun, Park Soo-Je, Rhee Sung-Keun
Department of Microbiology, Chungbuk National University, Cheongju, 362-763, Republic of Korea.
Antonie Van Leeuwenhoek. 2015 Apr;107(4):1049-56. doi: 10.1007/s10482-015-0396-4. Epub 2015 Jan 31.
A Gram-stain negative, long rod shaped, facultatively anaerobic bacterium, designated strain F2(T), was isolated from coastal sediment of the Korean Peninsula. Strain F2(T) was found to grow at 10-40 °C (optimum 30 °C), at pH 6.0-8.5 (optimum pH 7.5) and at 0.0-8.0 % (w/v) NaCl (optimum 3.0 %). Phylogenetic analysis of the 16S rRNA gene sequence showed that strain F2(T) is closely related to Draconibacterium orientale FH5(T) (with 97.9 % 16S rRNA gene similarity) of the family Prolixibacteraceae of the phylum Bacteroidetes. The major isoprenoid quinone was identified as MK-7 and the main fatty acids as iso-C15:0 (24.1 %), anteiso-C15:0 (15.4 %), C16:0 (10.7 %), iso-C17:0 3-OH (7.6 %) and iso-C16:0 3-OH (5.9 %). The major polar lipids were identified as phosphatidylethanolamine, phosphatidylinositol and four unidentified polar lipids. The genomic DNA G+C content of strain F2(T) was determined to be 44.7 mol% and the DNA-DNA relatedness of strain F2(T) with D. orientale DSM 25947(T) was 34.6 ± 4.3 %. Nitrate reduction capability and cell morphology of strain F2(T) are distinct from those of the closest relative, D. orientale DSM 25947(T). Based on these properties, we propose strain F2(T) represents a novel species within the genus Draconibacterium, with the name Draconibacterium filum sp. nov. The type strain of D. filum is F2(T) (=KCTC 32486(T) = JCM 19986(T)).
从朝鲜半岛沿海沉积物中分离出一株革兰氏染色阴性、长杆状、兼性厌氧细菌，命名为菌株F2(T)。发现菌株F2(T)在10-40°C（最适温度30°C）、pH 6.0-8.5（最适pH 7.5）和0.0-8.0%（w/v）NaCl（最适3.0%）条件下生长。对16S rRNA基因序列的系统发育分析表明，菌株F2(T)与拟杆菌门Prolixibacteraceae科的东方龙杆菌FH5(T)密切相关（16S rRNA基因相似性为97.9%）。主要类异戊二烯醌被鉴定为MK-7，主要脂肪酸为异-C15:0（24.1%）、anteiso-C15:0（15.4%）、C16:0（10.7%）、异-C17:0 3-OH（7.6%）和异-C16:0 3-OH（5.9%）。主要极性脂类被鉴定为磷脂酰乙醇胺、磷脂酰肌醇和四种未鉴定的极性脂类。菌株F2(T)的基因组DNA G+C含量测定为44.7 mol%，菌株F2(T)与东方龙杆菌DSM 25947(T)的DNA-DNA相关性为34.6±4.3%。菌株F2(T)的硝酸盐还原能力和细胞形态与最接近的亲缘种东方龙杆菌DSM 25947(T)不同。基于这些特性，我们提议菌株F2(T)代表龙杆菌属内的一个新物种，命名为丝状龙杆菌（Draconibacterium filum sp. nov.）。丝状龙杆菌的模式菌株为F2(T)（=KCTC 32486(T)=JCM 19986(T)）。
