Hu Bing, Yang Qian, Cai Man, Tang Yue-Qin, Zhao Gui-Fang, Wu Xiao-Lei
College of Engineering, Peking University, Beijing, 100871, People's Republic of China.
Antonie Van Leeuwenhoek. 2015 Mar;107(3):663-73. doi: 10.1007/s10482-014-0361-7. Epub 2014 Dec 21.
Four novel Gram-stain-negative, rod-shaped, and non-motile bacterial strains, SLG210-21(T), SLG210-4, SLG210-5 and SLG210-14, were isolated from oil-contaminated saline soil in Shengli Oilfield, China. Growth were observed at 25-42 °C (optimum 37 °C), in the presence of 0-10 % (w/v) NaCl (optimum 0-1 %) and at pH 4.0-10.0 (optimum pH 7.6-8.6). All the strains were positive for catalase and α, β-galactosidase activities and nitrogen reduction, and negative for oxidase activity, glucose fermentation and hydrolysis of agar, starch, gelatin, Tween 40, 60 and 80. The DNA G+C contents of the four strains were 41.3-43.0 mol% and the predominant respiratory quinones were all menaquinone-7. The major fatty acids were iso-C15:0, anteiso-C15:0, summed feature 3 (C16:1 ω7c and/or C16:1 ω6c), C16:1 ω5c and summed feature 9 (iso-C17:1 ω9c and/or 10-methyl C16:0), while the polar lipids consisted of phosphatidylcholine, phosphatidylethanolamine, glycolipid, two unidentified phospholipids and two unidentified amino lipids. Phylogenetic analysis based on 16S rRNA gene sequences indicated that the four strains clustered together to form a stable branch in the family Cyclobacteriaceae, and were most closely related to the genera Cyclobacterium and Echinicola with the 16S rRNA gene sequence similarities being 88.6-90.3 and 89.6-91.4 %, respectively. DNA-DNA hybridization between SLG210-21(T) and the other three strains showed the relatedness of 93.8 ± 4.5, 96.2 ± 4.2 and 82.3 ± 4.8 %, respectively. Based on the polyphasic analysis, a novel species in a new genus, Negadavirga Shengliensis gen. nov., sp. nov., is proposed with SLG210-21(T) (=LMG 27737(T) = CGMCC1.12768(T)) [corrected] as the type strain.
从中国胜利油田受石油污染的盐渍土中分离出四株新型革兰氏阴性、杆状且无运动性的细菌菌株，分别为SLG210 - 21(T)、SLG210 - 4、SLG210 - 5和SLG210 - 14。在25 - 42℃（最适温度37℃）、0 - 10%（w/v）NaCl（最适浓度0 - 1%）以及pH 4.0 - 10.0（最适pH 7.6 - 8.6）条件下可观察到菌株生长。所有菌株的过氧化氢酶、α,β - 半乳糖苷酶活性及氮还原反应均呈阳性，而氧化酶活性、葡萄糖发酵以及琼脂、淀粉、明胶、吐温40、60和80的水解反应均呈阴性。这四株菌株的DNA G + C含量为41.3 - 43.0 mol%，主要呼吸醌均为甲基萘醌 - 7。主要脂肪酸为异C15:0、anteiso - C15:0、总和特征3（C16:1 ω7c和/或C16:1 ω6c）、C16:1 ω5c以及总和特征9（异C17:1 ω9c和/或10 - 甲基C16:0），极性脂质由磷脂酰胆碱、磷脂酰乙醇胺、糖脂、两种未鉴定的磷脂和两种未鉴定的氨基脂质组成。基于16S rRNA基因序列的系统发育分析表明，这四株菌株聚集在一起，在环杆菌科中形成一个稳定分支，与环杆菌属和棘杆菌属关系最为密切，16S rRNA基因序列相似性分别为88.6 - 90.3%和89.6 - 91.4%。SLG210 - 21(T)与其他三株菌株之间的DNA - DNA杂交显示相关性分别为93.8 ± 4.5%、96.2 ± 4.2%和82.3 ± 4.8%。基于多相分析，提出一个新属中的新物种，即胜利负向病毒属（Negadavirga Shengliensis），模式菌株为SLG210 - 21(T)（=LMG 27737(T) = CGMCC1.12768(T)）[已校正] 。
