Feng David, Lau Chris, Ng Lydia, Li Yang, Kuan Leonard, Sunkin Susan M, Dang Chinh, Hawrylycz Michael
Allen Institute for Brain Science, Seattle, WA 98103, United States.
Methods. 2015 Feb;73:90-7. doi: 10.1016/j.ymeth.2015.01.009. Epub 2015 Jan 27.
The Allen Mouse Brain Connectivity Atlas is a mesoscale whole brain axonal projection atlas of the C57Bl/6J mouse brain. All data were aligned to a common template in 3D space to generate a comprehensive and quantitative database of inter-areal and cell-type-specific projections. A suite of computational tools were developed to search and visualize the projection labeling experiments, available at http://connectivity.brain-map.org. We present three use cases illustrating how these publicly-available tools can be used to perform analyses of long range brain region connectivity. The use cases make extensive use of advanced visualization tools integrated with the atlas including projection density histograms, 3D computed anterograde and retrograde projection paths, and multi-specimen projection composites. These tools offer convenient access to detailed axonal projection information in the adult mouse brain and the ability to perform data analysis and visualization of projection fields and neuroanatomy in an integrated manner.
艾伦小鼠脑连接图谱是C57Bl/6J小鼠脑的中尺度全脑轴突投射图谱。所有数据均在三维空间中与一个通用模板对齐，以生成一个全面的、特定区域和细胞类型的投射定量数据库。开发了一套计算工具来搜索和可视化投射标记实验，可在http://connectivity.brain-map.org上获取。我们展示了三个用例，说明如何使用这些公开可用的工具来进行远程脑区连接性分析。这些用例大量使用了与图谱集成的先进可视化工具，包括投射密度直方图、三维计算的顺行和逆行投射路径，以及多样本投射合成图。这些工具提供了便捷的途径来获取成年小鼠脑中详细的轴突投射信息，并能够以综合方式对投射场和神经解剖结构进行数据分析和可视化。
