用于递送甲氨蝶呤的自组装溶菌酶/羧甲基纤维素纳米凝胶
Self-assembled lysozyme/carboxymethylcellulose nanogels for delivery of methotrexate.
作者信息
Li Zhenshun, Xu Wei, Zhang Chunlan, Chen Yijie, Li Bin
机构信息
College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, China; College of Life Science, Yangtze University, Jingzhou 434025, China; Jingchu Food Research and Development Center, Yangtze University, Jingzhou 434025, China.
College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, China.
出版信息
Int J Biol Macromol. 2015 Apr;75:166-72. doi: 10.1016/j.ijbiomac.2015.01.033. Epub 2015 Jan 28.
Nanogels (NGs) were fabricated with lysozyme and carboxymethylcellulose via a green self-assembly method. The prepared NGs were characterized by dynamic light scattering (DLS), zeta potential, Fourier transform infrared spectroscopy (FTIR) and transmission electron microscopy (TEM). Pyrene and isothiocyanate were introduced as fluorescent probes to research the hydrophobic area of the NGs and cells endocytosis, respectively. Methotrexate (MTX) was used to investigate the drug encapsulation property of the NGs. It turned out to be that the drug loaded NGs were regular spherical shape with a hydrodynamic diameter of about 123 nm. The drug loading efficiency was about 14.2%. The NGs can slowly release the drug and increase the bioavailability of the loaded drug. The NGs are promising carriers for the delivery of drugs and other bioactive molecules.
通过绿色自组装方法，用溶菌酶和羧甲基纤维素制备了纳米凝胶（NGs）。通过动态光散射（DLS）、ζ电位、傅里叶变换红外光谱（FTIR）和透射电子显微镜（TEM）对制备的NGs进行了表征。分别引入芘和异硫氰酸酯作为荧光探针，研究NGs的疏水区域和细胞内吞作用。使用甲氨蝶呤（MTX）研究NGs的药物包封性能。结果表明，载药的NGs呈规则球形，流体动力学直径约为123nm。载药效率约为14.2%。NGs可以缓慢释放药物并提高所载药物的生物利用度。NGs是用于递送药物和其他生物活性分子的有前景的载体。
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