Bardajee Ghasem Rezanejade, Hooshyar Zari, Farsi Maryam, Mobini Akram, Sang Golnaz
Department of Chemistry, Payame Noor University, PO BOX 19395-3697, Tehran, Iran.
Department of Chemistry, Payame Noor University, PO BOX 19395-3697, Tehran, Iran.
Mater Sci Eng C Mater Biol Appl. 2017 Mar 1;72:558-565. doi: 10.1016/j.msec.2016.11.109. Epub 2016 Nov 30.
Nanogels (NGs) are three-dimensional water soluble cross-linked hydrogel materials in the nanoscale size range with a high loading capacity for guest molecules and act as drug carrier systems. In the present work, a new type of thermo/pH sensitive NG comprising salep modified graphene oxide (SMGO) with branched N-isopropylacrylamide (NIPAM) and acrylic acid (AA) was prepared. The SMGO/P(NIPAM-co-AA) NGs exhibited nanoporous structure and spherical particles with diameters about 82nm as characterized by scanning electron microscopy (SEM), transmission electron microscopy (TEM), and dynamic light scattering (DLS). The samples were also characterized by Fourier transform infrared spectroscopy (FT-IR) and thermo gravimetric analysis (TGA) to further confirm about the formation of NGs. Doxorubicin (DOX) loaded SMGO/P(NIPAM-co-AA) NGs showed thermo/pH dependent releasing behavior: slow drug release at neutral pH and lower temperature but increased significantly in acidic pH and higher temperature, without any burst release. In addition, the NGs exhibited no effect on the cell viability in the tested concentration range up to 410μg/mL and drug release systems enhanced toxicity to HeLa cells when compared to the equivalent dose of the free drug. Overall, our results put forth NGs as potential candidates in the development of a new nanocarrier for anti-cancer drug delivery.
纳米凝胶（NGs）是纳米级尺寸范围内的三维水溶性交联水凝胶材料，对客体分子具有高负载能力，并作为药物载体系统。在本研究中，制备了一种新型的包含莎草粉改性氧化石墨烯（SMGO）、支链N-异丙基丙烯酰胺（NIPAM）和丙烯酸（AA）的热/ pH敏感纳米凝胶。扫描电子显微镜（SEM）、透射电子显微镜（TEM）和动态光散射（DLS）表征结果显示，SMGO / P（NIPAM-co-AA）纳米凝胶呈现纳米多孔结构和直径约82nm的球形颗粒。还通过傅里叶变换红外光谱（FT-IR）和热重分析（TGA）对样品进行了表征，以进一步确认纳米凝胶的形成。负载阿霉素（DOX）的SMGO / P（NIPAM-co-AA）纳米凝胶表现出热/ pH依赖性释放行为：在中性pH和较低温度下药物释放缓慢，但在酸性pH和较高温度下显著增加，且无任何突释现象。此外，在高达410μg / mL的测试浓度范围内，纳米凝胶对细胞活力没有影响，并且与等量的游离药物相比，药物释放系统增强了对HeLa细胞的毒性。总体而言，我们的研究结果表明纳米凝胶是开发新型抗癌药物递送纳米载体的潜在候选材料。
