Ryan Una, Paparini Andrea, Tong Kaising, Yang Rongchang, Gibson-Kueh Susan, O'Hara Amanda, Lymbery Alan, Xiao Lihua
School of Veterinary and Life Sciences, Murdoch University, Murdoch, Perth, Western Australia 6150, Australia.
School of Veterinary and Life Sciences, Murdoch University, Murdoch, Perth, Western Australia 6150, Australia.
Exp Parasitol. 2015 Mar;150:31-5. doi: 10.1016/j.exppara.2015.01.009. Epub 2015 Jan 28.
The morphological, biological, and molecular characteristics of Cryptosporidium piscine genotype 1 from the guppy (Poecilia reticulata) are described, and the species name Cryptosporidium huwi n. sp. is proposed to reflect its genetic and biological differences from gastric and intestinal Cryptosporidium species. Oocysts of C.huwi n. sp. over-lap in size with Cryptosporidium molnari, measuring approximately 4.4-4.9 µm (mean 4.6) by 4.0-4.8 µm (mean 4.4 µm) with a length to width ratio of 1.04 (0.92-1.35) (n = 50). Similar to C.molnari, C.huwi n. sp. was identified in the stomach only and clusters of oogonial and sporogonial stages were identified deep within the epithelium. However, phylogenetic analysis of 18S rRNA sequences indicated that C. huwi n. sp. exhibited 8.5-9.2% and 3.5% genetic distance from C.molnari isolates and piscine genotype 7 respectively. At the actin locus, the genetic distance between C.huwi n. sp. and C.molnari was 16.6%. The genetic distance between C.huwi n. sp. and other Cryptosporidium species at the 18S locus was 13.2%-17% and at the actin locus was 18.9%-26.3%. Therefore C. huwi n. sp. is genetically distinct from previously described Cryptosporidium species.
描述了孔雀鱼（Poecilia reticulata）中隐孢子虫鱼类基因型1的形态、生物学和分子特征，并提出了新物种名称隐孢子虫胡维（Cryptosporidium huwi n. sp.），以反映其与胃和肠道隐孢子虫物种在遗传和生物学上的差异。胡维隐孢子虫新种的卵囊大小与莫氏隐孢子虫（Cryptosporidium molnari）重叠，长约4.4 - 4.9微米（平均4.6微米），宽4.0 - 4.8微米（平均4.4微米），长宽比为1.04（0.92 - 1.35）（n = 50）。与莫氏隐孢子虫相似，胡维隐孢子虫新种仅在胃中被发现，在上皮细胞深处发现了卵原细胞和孢子生殖阶段的簇。然而，18S rRNA序列的系统发育分析表明，胡维隐孢子虫新种与莫氏隐孢子虫分离株和鱼类基因型7的遗传距离分别为8.5 - 9.2%和3.5%。在肌动蛋白基因座上，胡维隐孢子虫新种与莫氏隐孢子虫的遗传距离为16.6%。胡维隐孢子虫新种与其他隐孢子虫物种在18S基因座的遗传距离为13.2% - 17%，在肌动蛋白基因座为18.9% - 2
