Certad Gabriela, Zahedi Alireza, Gantois Nausicaa, Sawant Manasi, Creusy Colette, Duval Erika, Benamrouz-Vanneste Sadia, Ryan Una, Viscogliosi Eric
Institut Pasteur de Lille, U1019-UMR 9017-CIIL-Centre d'Infection et d'Immunité de Lille, Université de Lille, CNRS, Inserm, CHU Lille, F-59000 Lille, France.
Délégation à la Recherche Clinique et à l'Innovation, Groupement des Hôpitaux de l'Institut Catholique de Lille, F-59462 Lomme, France.
Microorganisms. 2020 Dec 16;8(12):2014. doi: 10.3390/microorganisms8122014.
Current knowledge of species/genotypes in marine fish is limited. Following phylogenetic analysis at the 18S rDNA locus, a recent study identified six new genotypes of colonizing edible fish found in European seas. Of these, five grouped in a clade together (#Cryptofish 1-5) and one grouped separately (#Cryptofish 7). In the present study, after phylogenetic analyses of #Cryptofish1, #Cryptofish2, #Cryptofish4, #Cryptofish5 and #Cryptofish7 at the actin locus, the presence of two major clades was confirmed. In addition, when possible, longer 18S amplicons were generated. In conclusion, the small genetic distances between these genotypes designated as a novel marine genotype I (#Cryptofish 1-5) suggest that they may be genetic variants of the same species, while the designated novel marine genotype 2 (#Cryptofish 7) is clearly representative of a separate species.
目前对海洋鱼类物种/基因型的了解有限。在对18S rDNA位点进行系统发育分析后，最近的一项研究在欧洲海域发现的可食用鱼类中鉴定出六种新的基因型。其中，五种聚集在一个进化枝中（#隐鱼1 - 5），一种单独聚集（#隐鱼7）。在本研究中，对#隐鱼1、#隐鱼2、#隐鱼4、#隐鱼5和#隐鱼7在肌动蛋白位点进行系统发育分析后，证实存在两个主要进化枝。此外，在可能的情况下，生成了更长的18S扩增子。总之，这些被指定为新型海洋基因型I（#隐鱼1 - 5）的基因型之间较小的遗传距离表明它们可能是同一物种的遗传变体，而指定的新型海洋基因型2（#隐鱼7）显然代表了一个单独的物种。
