Huang Yong, Li Shasha, Zhao Qiumin, Pei Guangqian, An Xiaoping, Guo Xiaofang, Zhou Hongning, Zhang Zhiyi, Zhang Jiusong, Tong Yigang
State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, 20 Dong-Da Street, Fengtai District, Beijing 100071, China.
Yunnan Institute of Parasitic Diseases, Pu'er, Yunnan, China.
J Invertebr Pathol. 2015 May;127:1-5. doi: 10.1016/j.jip.2015.01.007. Epub 2015 Jan 28.
An invertebrate iridovirus (designated AMIV) was isolated from adult wild-captured Anopheles minimus mosquitoes in China. AMIV was pathologically and morphologically characterized and sequenced using the Ion Torrent™ sequencing platform. Phylogenetic analysis based on both the major capsid protein and core genes revealed that AMIV differs from all the members of the family Iridoviridae. The AMIV negatively strained virion has a diameter of about 130nm. AMIV contains a linear DNA molecule of 163,023bp, with 39% G+C content and 148 coding sequences. The genome analysis revealed that AMIV genome encodes a high content of replication associated genes including BRO-like genes. This is the ninth complete genome of IIV reported.
从中国野外捕获的成年微小按蚊中分离出一种无脊椎动物虹彩病毒（命名为AMIV）。利用Ion Torrent™测序平台对AMIV进行了病理、形态学特征分析及测序。基于主要衣壳蛋白和核心基因的系统发育分析表明，AMIV不同于虹彩病毒科的所有成员。AMIV负链病毒粒子直径约为130nm。AMIV含有一个163,023bp的线性DNA分子，G+C含量为39%，有148个编码序列。基因组分析显示，AMIV基因组编码了高含量的与复制相关的基因，包括类BRO基因。这是报道的第九个虹彩病毒属完整基因组。
