Cheng Tian, Yang Bo, Li Dongpeng, Ma Shanshan, Tian Yi, Qu Ruina, Zhang Wenjin, Zhang Yanting, Hu Kai, Guan Fangxia, Wang Jian
The First Affiliated Hospital of Zhengzhou University, Zhengzhou, 450052, Henan, People's Republic of China.
Cell Mol Neurobiol. 2015 Jul;35(5):641-9. doi: 10.1007/s10571-015-0159-9. Epub 2015 Feb 1.
Traumatic brain injury (TBI), which can lead to disability, dysfunction, and even death, is a prominent health problem worldwide. Effective therapy for this serious and debilitating condition is needed. Human umbilical cord matrix, known as Wharton's jelly (WJ), provides a natural, interface scaffold that is enriched in mesenchymal stem cells. In this study, we tested the efficacy of WJ tissue transplantation in a weight-drop model of TBI in rats. WJ tissue was cultured and transplanted into the injury site 24 h after TBI. The modified neurologic severity score, body weight, brain edema, and lesion volume were evaluated at various time points after TBI. Cognitive behavior was assessed by the novel object recognition test and the Morris water maze test. Expression of brain-derived neurotrophic factor (BDNF) in the perilesional brain area was measured at day 14 after TBI. We found that WJ tissue transplantation lessened TBI-induced brain edema (day 3), reduced lesion volume (day 28), improved neurologic function (days 21-28), and promoted memory and cognitive recovery. Additionally, expression of BDNF mRNA and protein was higher in WJ tissue-treated rats than in sham-operated or vehicle-treated rats. These data suggest that WJ tissue transplantation can reduce TBI-induced brain injury and may have therapeutic potential for the treatment of TBI.
创伤性脑损伤（TBI）可导致残疾、功能障碍甚至死亡，是全球一个突出的健康问题。需要针对这种严重且使人衰弱的病症的有效治疗方法。人脐带基质，即华通氏胶（WJ），提供了一种富含间充质干细胞的天然界面支架。在本研究中，我们在大鼠TBI的重物坠落模型中测试了WJ组织移植的疗效。WJ组织经培养后在TBI后24小时移植到损伤部位。在TBI后的不同时间点评估改良神经功能严重程度评分、体重、脑水肿和损伤体积。通过新物体识别测试和莫里斯水迷宫测试评估认知行为。在TBI后第14天测量损伤周围脑区脑源性神经营养因子（BDNF）的表达。我们发现WJ组织移植减轻了TBI诱导的脑水肿（第3天），减小了损伤体积（第28天），改善了神经功能（第21 - 28天），并促进了记忆和认知恢复。此外，WJ组织治疗的大鼠中BDNF mRNA和蛋白的表达高于假手术或载体治疗的大鼠。这些数据表明WJ组织移植可减轻TBI诱导的脑损伤，可能对TBI的治疗具有治疗潜力。
