Wu Lei, Xu Liangliang, Hu Chunxiang
Key Laboratory of Algal Biology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, P.R. China.
University of Chinese Academy of Sciences, Beijing 100039, P.R. China.
J Microbiol Biotechnol. 2015 Jun;25(6):910-7. doi: 10.4014/jmb.1411.11075.
A total of 646 strains, including green algae and diatoms, were isolated from 220 samples to screen microalgae with high lipid productivity (LP). The samples were obtained from nine habitats in Northern Xinjiang, China in June 2013. This study initially identified eight lipidrich strains, namely, Desmodesmus intermedius XJ-498, D. intermedius XJ-145, D. intermedius XJ- 99, Monoraphidium pusillum XJ-489, M. dybowskii XJ-435, M. dybowskii XJ-151, Mychonastes homosphaera XJ-488, and Podohedriella falcata XJ-176, based on 18S rDNA sequencing. The strains were cultured in a photobioreactor for the same period. Results showed that the specific growth rate (day(-1)) of M. pusillum XJ-489 was the highest (1.14 ± 0.06), and the biomass concentration (g/l) of D. intermedius XJ-99 was the highest (2.84 ± 0.3). Futhermore, the lipid content (%) of M. dybowskii XJ-151 was the highest (33.5 ± 4.38), and the lipid productivity (mg l(-1) day(-1)) of My. homosphaera XJ-488 was the highest (86.41 ± 9.04). C16 to C18 accounted for 86% to 98% of the total lipid, and the biodiesel qualities of the selected algae corresponded to international standards. This study suggests that My. homosphaera XJ-488, D. intermedius XJ-99, and M. dybowskii XJ-151 are the most potential strains for biodiesel production among all the isolated strains.
从220个样本中总共分离出646株藻类，包括绿藻和硅藻，以筛选具有高脂质生产率（LP）的微藻。这些样本于2013年6月取自中国新疆北部的九个栖息地。本研究最初基于18S rDNA测序鉴定出八株富含脂质的菌株，即中间栅藻XJ - 498、中间栅藻XJ - 145、中间栅藻XJ - 99、微小单针藻XJ - 489、迪氏单针藻XJ - 435、迪氏单针藻XJ - 151、等球藻XJ - 488和镰形足孔藻XJ - 176。这些菌株在光生物反应器中培养相同时间。结果表明，微小单针藻XJ - 489的比生长速率（天⁻¹）最高（1.14±0.06），中间栅藻XJ - 99的生物量浓度（克/升）最高（2.84±0.3）。此外，迪氏单针藻XJ - 151的脂质含量（%）最高（33.5±4.38），等球藻XJ - 488的脂质生产率（毫克/升·天⁻¹）最高（86.41±9.04）。C16至C18占总脂质的86%至98%，所选藻类的生物柴油质量符合国际标准。本研究表明，在所有分离菌株中，等球藻XJ - 488、中间栅藻XJ - 99和迪氏单针藻XJ - 151是最具生物柴油生产潜力的菌株。
