Fox Cade B, Kim Jean, Schlesinger Erica B, Chirra Hariharasudhan D, Desai Tejal A
Department of Bioengineering and Therapeutic Sciences, University of California , San Francisco, California 94158, United States.
Nano Lett. 2015 Mar 11;15(3):1540-6. doi: 10.1021/nl503872p. Epub 2015 Feb 5.
Herein, we present a novel approach for the fabrication of micropatterned polymeric nanowire arrays that addresses the current need for scalable and customizable polymer nanofabrication. We describe two variations of this approach for the patterning of nanowire arrays on either flat polymeric films or discrete polymeric microstructures and go on to investigate biological applications for the resulting polymeric features. We demonstrate that the micropatterned arrays of densely packed nanowires facilitate rapid, low-waste drug and reagent localization with micron-scale resolution as a result of their high wettability. We also show that micropatterned nanowire arrays provide hierarchical cellular control by simultaneously directing cell shape on the micron scale and influencing focal adhesion formation on the nanoscale. This nanofabrication approach has potential applications in scaffold-based cellular control, biological assay miniaturization, and biomedical microdevice technology.
在此，我们提出了一种制造微图案化聚合物纳米线阵列的新方法，该方法满足了当前对可扩展且可定制的聚合物纳米制造的需求。我们描述了这种方法的两种变体，用于在平坦的聚合物薄膜或离散的聚合物微结构上对纳米线阵列进行图案化，并继续研究所得聚合物特征的生物学应用。我们证明，由于其高润湿性，密集排列的纳米线微图案化阵列有助于以微米级分辨率实现快速、低浪费的药物和试剂定位。我们还表明，微图案化纳米线阵列通过同时在微米尺度上引导细胞形状和在纳米尺度上影响粘着斑形成，提供了分级细胞控制。这种纳米制造方法在基于支架的细胞控制、生物测定小型化和生物医学微器件技术方面具有潜在应用。
