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谷氨酰胺合成酶在人肝脏中的免疫组织化学定位

Immunohistochemical localization of glutamine synthetase in human liver.

作者信息

Gebhardt R, Schmid H, Fitzke H

机构信息

Physiologisch-chemisches Institut, Tübingen, Federal Republic of Germany.

出版信息

Experientia. 1989 Feb 15;45(2):137-9. doi: 10.1007/BF01954848.

DOI:10.1007/BF01954848
PMID:2563977
Abstract

Glutamine synthetase (GS) of human liver was recognized with a polyclonal antibody to pig brain GS, but failed to stain with an antibody against rat liver GS. Using the latter antibody GS of human liver was shown to be localized within small rings of 1 to 3 hepatocytes surrounding the terminal hepatic venules. This pattern was analogous to that seen in rat and mouse liver.

摘要

用人脑谷氨酰胺合成酶(GS)的多克隆抗体可识别出人类肝脏中的谷氨酰胺合成酶,但用抗大鼠肝脏GS的抗体进行染色时却未成功。使用后一种抗体显示,人类肝脏中的GS定位于围绕终末肝静脉的1至3个肝细胞的小环内。这种模式与在大鼠和小鼠肝脏中观察到的模式类似。

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本文引用的文献

1
Heterogeneous distribution of glutamine synthetase among rat liver parenchymal cells in situ and in primary culture.谷氨酰胺合成酶在大鼠肝脏实质细胞原位及原代培养中的异质性分布。
EMBO J. 1983;2(4):567-70. doi: 10.1002/j.1460-2075.1983.tb01464.x.
2
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.在噬菌体T4头部组装过程中结构蛋白的切割
Nature. 1970 Aug 15;227(5259):680-5. doi: 10.1038/227680a0.
3
Alterations of hepatic enzyme levels and of the acinar distribution of glutamine synthetase in response to experimental liver injury in the rat.
大鼠实验性肝损伤后肝酶水平及谷氨酰胺合成酶腺泡分布的变化
Hepatology. 1988 Jul-Aug;8(4):822-30. doi: 10.1002/hep.1840080421.
4
Distribution of glutamine synthetase and carbamoyl-phosphate synthetase I in vertebrate liver.谷氨酰胺合成酶和氨甲酰磷酸合成酶I在脊椎动物肝脏中的分布
Proc Natl Acad Sci U S A. 1988 Jan;85(1):160-4. doi: 10.1073/pnas.85.1.160.
5
Acquisition of antigens characteristic of adult pericentral hepatocytes by differentiating fetal hepatoblasts in vitro.体外分化的胎儿肝母细胞获取成年肝小叶中央周围肝细胞的特征性抗原。
J Cell Biol. 1987 Sep;105(3):1073-85. doi: 10.1083/jcb.105.3.1073.
6
Reciprocal regulation of glutamine synthetase and carbamoylphosphate synthetase levels in rat liver.大鼠肝脏中谷氨酰胺合成酶和氨甲酰磷酸合成酶水平的相互调节
Biochim Biophys Acta. 1987 Apr 29;908(3):231-40. doi: 10.1016/0167-4781(87)90103-5.
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Specific chromosomal abnormalities characterize four established cell lines derived from malignant human gliomas.特定的染色体异常是源自人类恶性胶质瘤的四种成熟细胞系的特征。
Acta Neuropathol. 1986;72(1):86-97. doi: 10.1007/BF00687952.
8
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9
Functional hepatocyte heterogeneity in ammonia metabolism. The intercellular glutamine cycle.氨代谢中功能性肝细胞的异质性。细胞间谷氨酰胺循环。
J Hepatol. 1985;1(1):3-14. doi: 10.1016/s0168-8278(85)80063-5.