Avantaggiato Anna, Girardi Ambra, Palmieri Annalisa, Pascali Michele, Carinci Francesco
1 Department of Morphology, Surgery and Experimental Medicine, University of Ferrara , Ferrara, Italy .
2 Department of Experimental, Diagnostic and Specialty Medicine, University of Bologna , Bologna, Italy .
Rejuvenation Res. 2015 Jun;18(3):270-6. doi: 10.1089/rej.2014.1654. Epub 2015 May 20.
Bio-revitalization is a commonly used technique in aesthetic medicine for improving skin quality and appearance by intra-dermal injection of hyaluronic acid (HA)-containing compounds. The present study compares different HA-containing injectables regarding their effects on cultured skin fibroblasts over time (24, 48, and 72 hr) by using RT-PCR and a panel of genes involved in dermal integrity. Human dermal fibroblasts were seeded on a layer of five different commercial medical devices containing 6.2 mg/mL 10 mg/mL 10%, 13 mg/mL and 20 mg/mL, respectively, of HA. The products differ not only in HA concentration but also in the content and quality of other ingredients; moreover, one of these products contained cross-linked HA. Differences among medical devices were found. In particular, HA concentration seems to be inversely correlated to elastin gene activation. Regarding the neutrophil elastase gene, the two medical devices with the higher concentration of HA displayed the greater effect. Genes encoding for hyaluronan synthase 1, hyaluronidase 1, and desmoplakin were enhanced, but the HA content of the different products did not seem to be directly related to gene activation. Therefore, the explanation for the differences must be studied further with respect to elements that are distinctive for each device. For the physician, it is important to choose which drugs or medical devices can be used and in what protocols. The present study performed a comparison that can be useful in better addressing the skin improvement therapies for aging and in its prevention.
生物修复是美容医学中一种常用技术，通过皮内注射含透明质酸（HA）的化合物来改善皮肤质量和外观。本研究通过逆转录聚合酶链反应（RT-PCR）以及一组与皮肤完整性相关的基因，比较了不同含HA注射剂在不同时间（24小时、48小时和72小时）对培养的皮肤成纤维细胞的影响。将人皮肤成纤维细胞接种在五层不同的商业医疗设备上，这些设备分别含有浓度为6.2毫克/毫升、10毫克/毫升、10%、13毫克/毫升和20毫克/毫升的HA。这些产品不仅HA浓度不同，其他成分的含量和质量也不同；此外，其中一种产品含有交联HA。研究发现了不同医疗设备之间的差异。特别是，HA浓度似乎与弹性蛋白基因激活呈负相关。关于中性粒细胞弹性蛋白酶基因，HA浓度较高的两种医疗设备显示出更大的效果。编码透明质酸合酶1、透明质酸酶1和桥粒斑蛋白的基因得到增强，但不同产品的HA含量似乎与基因激活没有直接关系。因此，必须进一步研究每个设备独特元素方面的差异原因。对于医生来说，重要的是选择可以使用哪些药物或医疗设备以及采用何种方案。本研究进行的比较有助于更好地应对衰老皮肤改善疗法及其预防。
