Mandrillo P M, Fischetto G, Odorisio P, Cura F, Avantaggiato A, Carinci F
Private practice, Taranto, Italy.
Private practice, Teramo, Italy.
Oral Implantol (Rome). 2017 Sep 27;10(2):151-161. doi: 10.11138/orl/2017.10.2.151. eCollection 2017 Apr-Jun.
The light-emitting diodes (LEDs) have been applied in oral surgery for tissue stimulation and wound healing. Several Authors have highlighted that fibroblasts subjected to phototherapy have an increased viability, proliferation, biomodulation of inflammatory cytokines and genes expression. It remains to be determined which are the best irradiation parameters (energy, wavelength, power) for each type of cell in order to obtain the best bio-stimulation. The aim of this study was to investigate the effects of LED irradiation on primary human gingival fibroblast cells (HGF) on DSP, ELN, HAS1, ELANE, HYAL1, RPL13 genes activation using Real Time PCR. These genes activation is directly connected with elastin protein production and HGF functionality.
Human gingival tissue biopsies were obtained from three healthy patients during extraction of teeth. The gingival pieces were fragmented with a scalpel and transferred in culture dishes for allow the cells growth. Human gingival fibroblasts at the second passage were seeded on multiple 6-well plates and were stimulated with three different light-emitting diodes (LEDs) fixture. After irradiation, the cells were trypsinized, harvested and lysed for RNA extraction. Genes expression was quantified using Real Time PCR.
We didn't found significant differences in genes activation of HGF of the three different LEDs. The LED irradiation seems to be directly correlated with the elastin and hyaluronoglucosaminidase 1 genes activation that are directly connected with proteins production and HGF functionality.
HGF show an increased deposition of elastin as well as enhanced expression of collagen type I, which is the main protein related to the synthesis and of the collagen-rich matrix.
发光二极管（LED）已应用于口腔外科手术，用于组织刺激和伤口愈合。几位作者强调，接受光疗的成纤维细胞具有更高的活力、增殖能力、炎症细胞因子的生物调节作用和基因表达。为了获得最佳的生物刺激效果，每种细胞类型的最佳照射参数（能量、波长、功率）仍有待确定。本研究的目的是使用实时聚合酶链反应（Real Time PCR）研究LED照射对原代人牙龈成纤维细胞（HGF）中DSP、ELN、HAS1、ELANE、HYAL1、RPL13基因激活的影响。这些基因的激活与弹性蛋白的产生和HGF的功能直接相关。
在拔牙过程中从三名健康患者身上获取人牙龈组织活检样本。用手术刀将牙龈组织切碎，转移至培养皿中以促进细胞生长。将第二代人牙龈成纤维细胞接种到多个6孔板中，并用三种不同的发光二极管（LED）装置进行刺激。照射后，将细胞用胰蛋白酶消化、收集并裂解以提取RNA。使用实时聚合酶链反应（Real Time PCR）对基因表达进行定量分析。
我们未发现三种不同LED照射下HGF的基因激活存在显著差异。LED照射似乎与弹性蛋白和透明质酸葡萄糖苷酶1基因的激活直接相关，而这两种基因又与蛋白质产生和HGF功能直接相关。
HGF显示出弹性蛋白沉积增加以及I型胶原蛋白表达增强，I型胶原蛋白是与富含胶原蛋白的基质合成相关的主要蛋白质。
