Yaqoob Mohammad, Asghar Mohammad, Nabi Abdul
Department of Chemistry, University of Balochistan, Sariab Road, Quetta, 87300, Pakistan.
Luminescence. 2015 Nov;30(7):950-61. doi: 10.1002/bio.2844. Epub 2015 Jan 30.
A flow-injection (FI) method is reported for the determination of Mn(II), maneb and mancozeb fungicides based on the catalytic effect of Mn(II) on the oxidation of lucigenin and dissolved oxygen in a basic solution. The Tween-20 surfactant has been reported for first time to enhance lucigenin chemiluminescence (CL) intensity in the presence of Mn(II) (53%) and maneb and mancozeb (89%). The calibration graphs were linear in the concentration range of 0.001-1.5 mg L(-1) (R(2) = 0.9982 (n = 11) with a limit of detection (S/N = 3) of 0.1 µg L(-1) for Mn(II) and 0.01-3.0 mg L(-1) [R(2) = 0.9989 and R(2) = 0.9992 (n = 6)] with a limit of detection (S/N =3) of 1.0 µg L(-1) for maneb and mancozeb respectively. Injection throughputs of 90 and 120 h(-1) for Mn(II) and maneb and mancozeb respectively, and relative standard deviations of 1.0-3.4% were obtained in the concentration range studied. The experimental variables, e.g., reagents concentrations, flow rates, sample volume, and photomultiplier tube voltage, were optimized and potential interferences were investigated. The analysis of Mn(II) in river water reference materials (SLRS-4 and SLRS-5) showed good agreement with the certified values incorporating an on-line 8-hydroxyquinoline chelating column in the manifold for removing interfering metal ions. Recoveries for maneb and mancozeb were in the range of 92 ± 5 to 104 ± 3% and 91 ± 2 to 100 ± 4% (n = 3) respectively. The effect of 30 other pesticides (fungicides, herbicides and insecticides) was also examined in the lucigenin-Tween-20 CL system.
报道了一种流动注射（FI）法，用于测定Mn（II）、代森锰和代森锰锌杀菌剂，该方法基于Mn（II）在碱性溶液中对光泽精氧化和溶解氧的催化作用。首次报道了吐温-20表面活性剂在Mn（II）（53%）以及代森锰和代森锰锌（89%）存在下可增强光泽精化学发光（CL）强度。校准曲线在0.001 - 1.5 mg L⁻¹浓度范围内呈线性（R² = 0.9982，n = 11），Mn（II）的检测限（S/N = 3）为0.1 μg L⁻¹；在0.01 - 3.0 mg L⁻¹范围内呈线性[R² = 0.9989和R² = 0.9992，n = 6]，代森锰和代森锰锌的检测限（S/N = 3）分别为1.0 μg L⁻¹。Mn（II）、代森锰和代森锰锌的进样通量分别为90和120 h⁻¹，在所研究的浓度范围内相对标准偏差为1.0 - 3.4%。对实验变量如试剂浓度、流速、样品体积和光电倍增管电压进行了优化，并研究了潜在干扰。对河水标准物质（SLRS - 4和SLRS - 5）中Mn（II）的分析表明，在流路中加入在线8 - 羟基喹啉螯合柱以去除干扰金属离子后，分析结果与认证值吻合良好。代森锰和代森锰锌的回收率分别在92 ± 5至104 ± 3%和91 ± 2至100 ± 4%（n = 3）范围内。还在光泽精 - 吐温 - 20 CL体系中研究了其他30种农药（杀菌剂、除草剂和杀虫剂）的影响。
