Department of Chemistry, University of Balochistan, Quetta, Pakistan.
Department of Chemistry, Sardar Bahadur Khan Women' University Quetta, Pakistan.
Luminescence. 2020 Feb;35(1):79-89. doi: 10.1002/bio.3700. Epub 2019 Aug 29.
Chemiluminescence (CL) of the rhodamine 6-G-diperiodatonickelate (IV) (Rh6-G-Ni(IV) complex) in the presence of Brij-35 was examined in an alkaline medium and implemented using flow-injection analysis to analyze Mn(II) in natural waters. Brij-35 was identified as the surfactant of choice that enhanced CL intensity by about 62% of the reaction. The calibration curves were linear in the range 1.7 × 10 - 0.2 (0.9990, n = 7) and 8.0 × 10 - 0.1 μg ml (0.9990, n = 7) with limits of detection (LODs) (S:N = 3) of 5.0 × 10 and 2.4 × 10 μg ml without and with using an in-line 8-hydroxyquinoline (8-HQ) resin mini-column, respectively. The sample throughput and relative standard deviation were 200 h and 1.7-2.2% in the range studied respectively. Mn(II) concentrations in certified reference materials and natural water samples was successfully determined. A brief discussion about the possible CL reaction mechanism is also given. In addition, analysis of V(III), Cr(III) and Fe(II) was also performed without and with using an in-line 8-HQ column and selective elution of each metal ion was achieved by adjusting the pH of the sample carrier stream with aqueous HCl solution.
碱性介质中，铼 6-G-双过碘酸盐镍（IV）（Rh6-G-Ni（IV）配合物）的化学发光（CL）在 Brij-35 的存在下被检测，并采用流动注射分析来分析天然水中的 Mn(II)。Brij-35 被确定为首选的表面活性剂，可将反应的 CL 强度增强约 62%。校准曲线在 1.7×10 - 0.2（0.9990，n=7）和 8.0×10 - 0.1μg ml（0.9990，n=7）范围内呈线性，检出限（LOD）（S：N=3）分别为 5.0×10 和 2.4×10μg ml，而无需和使用在线 8-羟基喹啉（8-HQ）树脂微型柱时，分别为 2.4×10μg ml。在研究范围内，样品通量和相对标准偏差分别为 200 h 和 1.7-2.2%。成功地测定了认证参考材料和天然水样中的 Mn(II)浓度。还对可能的 CL 反应机制进行了简要讨论。此外，还进行了 V(III)、Cr(III)和 Fe(II)的分析，无需和使用在线 8-HQ 柱，通过用稀盐酸溶液调节样品载体流的 pH 值，实现了每种金属离子的选择性洗脱。
