Ayyildiz Talat, Dolar Enver, Ugras Nesrin, Eminler Ahmet Tarik, Erturk Banu, Adim Saduman Balaban, Yerci Omer
Department of Gastroenterology, Medical Faculty, Ondokuz Mayis University, Samsun, Turkey E-mail :
Asian Pac J Cancer Prev. 2015;16(1):367-72. doi: 10.7314/apjcp.2015.16.1.367.
Human adiponectin (ApN), a 30 kDa glycoprotein of 244-amino acids which is predominantly produced by adipocytes, exerts its effects via two receptors, namely adiponectin receptor-1 (adipo-R1) and adiponectin receptor-2 (adipo-R2) with differential binding affinity to globular adiponectin. Adiponectin receptor expression has been studied in several cancer tissues. However, there are no studies of colorectal adenomas which are considered to be precursors for colorectal carcinoma (CRC).
In the present study, the expression of adipo-R1 and adipo-R2 was investigated immunohistochemically in colorectal adenomas and colorectal carcinoma tissues in an attempt to determine associations with these tumors.
The study enrolled 50 CRC patients with tumor resection and 82 patients who were diagnosed with adenomatous polyps, classified as negative for neoplasia, low-grade dysplasia (L-GD) or high- grade dysplasia (H-GD).
Expression of both adipo-R1 and adipo-R2 was found to be significantly lower in the CRCs than in colorectal adenomas (tubular and tubulovillous, p=0.009 and p<0.001, respectively). Adipo-R1 and adipo-R2 expression was also significantly lower in the CRC group when compared with the groups of patients with low grade dysplasia, high-grade dysplasia or no neoplasia (p=0.012 and p<0.001, respectively). In addition, it was observed that adipo-R2 expression was generally positive in the non-neoplastic group irrespective of the adipo-R2 expression. In the L-GD, H-GD and CRC groups, the adipo-R2 result was positive whenever adipo-R1 result was positive but some patients with negative adipo-R1 had positive adipo-R2 (p<0.001, p=0.004, p<0.001, respectively).
This study indicated that ApN may play a role in the progression of colorectal adenomatous polyps to carcinoma through actions on adipo-R1 and adipo-R2 receptors.
人脂联素（ApN）是一种由244个氨基酸组成的30 kDa糖蛋白，主要由脂肪细胞产生，它通过两种受体发挥作用，即脂联素受体1（adipo - R1）和脂联素受体2（adipo - R2），这两种受体对球状脂联素具有不同的结合亲和力。脂联素受体的表达已在多种癌症组织中进行了研究。然而，尚无关于被认为是结直肠癌（CRC）前体的大肠腺瘤的研究。
在本研究中，采用免疫组织化学方法研究脂联素受体1（adipo - R1）和脂联素受体2（adipo - R2）在大肠腺瘤和结直肠癌组织中的表达情况，以确定其与这些肿瘤的相关性。
本研究纳入了50例接受肿瘤切除术的CRC患者以及82例被诊断为腺瘤性息肉的患者，这些患者被分类为无肿瘤、低级别发育异常（L - GD）或高级别发育异常（H - GD）。
发现CRC中脂联素受体1（adipo - R1）和脂联素受体2（adipo - R2）的表达均显著低于大肠腺瘤（管状腺瘤和管状绒毛状腺瘤，p分别为0.009和p < 0.001）。与低级别发育异常、高级别发育异常或无肿瘤的患者组相比，CRC组中脂联素受体1（adipo - R1）和脂联素受体2（adipo - R2）的表达也显著降低（p分别为0.012和p < 0.001）。此外，观察到在非肿瘤组中，无论脂联素受体2（adipo - R2）的表达如何，其表达通常为阳性。在低级别发育异常（L - GD）、高级别发育异常（H - GD）和CRC组中，当脂联素受体1（adipo - R1）结果为阳性时，脂联素受体2（adipo - R2）结果均为阳性，但一些脂联素受体1（adipo - R1）为阴性的患者脂联素受体2（adipo - R2）为阳性（p分别为< 0.001、0.004、< 0.001）。
本研究表明，ApN可能通过作用于脂联素受体1（adipo - R1）和脂联素受体2（adipo - R2）在大肠腺瘤性息肉向癌的进展过程中发挥作用。
