Sun Linjie, Wang Yan, Song Yu, Cheng Xiang-Rong, Xia Shufang, Rahman Md Ramim Tanver, Shi Yonghui, Le Guowei
The State Key Laboratory of Food Science and Technology, Research Center of Food Nutrition and Functional Factors, School of Food Science and Technology, Jiangnan University, Wuxi 214122, China.
The State Key Laboratory of Food Science and Technology, Research Center of Food Nutrition and Functional Factors, School of Food Science and Technology, Jiangnan University, Wuxi 214122, China.
Biochem Biophys Res Commun. 2015 Feb 27;458(1):86-91. doi: 10.1016/j.bbrc.2015.01.072. Epub 2015 Jan 29.
Circadian rhythmic disorders induced by high-fat diet are associated with metabolic diseases. Resveratrol could improve metabolic disorder, but few reports focused on its effects on circadian rhythm disorders in a variety of studies. The aim of the present study was to analyze the potential effects of resveratrol on high-fat diet-induced disorders about the rhythmic expression of clock genes and clock-controlled lipid metabolism. Male C57BL/6 mice were divided into three groups: a standard diet control group (CON), a high-fat diet (HFD) group and HFD supplemented with 0.1% (w/w) resveratrol (RES). The body weight, fasting blood glucose and insulin, plasma lipids and leptin, whole body metabolic status and the expression of clock genes and clock-controlled lipogenic genes were analyzed at four different time points throughout a 24-h cycle (8:00, 14:00, 20:00, 2:00). Resveratrol, being associated with rhythmic restoration of fasting blood glucose and plasma insulin, significantly decreased the body weight in HFD mice after 11 weeks of feeding, as well as ameliorated the rhythmities of plasma leptin, lipid profiles and whole body metabolic status (respiratory exchange ratio, locomotor activity, and heat production). Meanwhile, resveratrol modified the rhythmic expression of clock genes (Clock, Bmal1 and Per2) and clock-controlled lipid metabolism related genes (Sirt1, Pparα, Srebp-1c, Acc1 and Fas). The response pattern of mRNA expression for Acc1 was similar to the plasma triglyceride. All these results indicated that resveratrol reduced lipogenesis and ultimately normalized rhythmic expression of plasma lipids, possibly via its action on clock machinery.
高脂饮食诱导的昼夜节律紊乱与代谢性疾病相关。白藜芦醇可改善代谢紊乱，但在众多研究中，很少有报道关注其对昼夜节律紊乱的影响。本研究的目的是分析白藜芦醇对高脂饮食诱导的生物钟基因节律性表达和生物钟控制的脂质代谢紊乱的潜在影响。将雄性C57BL/6小鼠分为三组：标准饮食对照组（CON）、高脂饮食（HFD）组和补充0.1%（w/w）白藜芦醇（RES）的高脂饮食组。在24小时周期的四个不同时间点（8:00、14:00、20:00、2:00）分析体重、空腹血糖和胰岛素、血脂和瘦素、全身代谢状态以及生物钟基因和生物钟控制的脂肪生成基因的表达。白藜芦醇与空腹血糖和血浆胰岛素的节律恢复相关，在喂食11周后显著降低了高脂饮食小鼠的体重，并改善了血浆瘦素、血脂谱和全身代谢状态（呼吸交换率、运动活动和产热）的节律性。同时，白藜芦醇改变了生物钟基因（Clock、Bmal1和Per2）以及生物钟控制的脂质代谢相关基因（Sirt1、Pparα、Srebp-1c、Acc1和Fas）的节律性表达。Acc1的mRNA表达反应模式与血浆甘油三酯相似。所有这些结果表明，白藜芦醇可能通过作用于生物钟机制减少脂肪生成并最终使血浆脂质的节律性表达正常化。
