Sun Dawei, Zheng Xiaochen, Chen Yuhui, Jia Chunhong, Xu Song, Lin Chuangxin, Zhang Pei, Zhang Zhongmin, Cai Daozhang, Jin Dadi, Zhang Baiyu, Bai Xiaochun
Department of Orthopedics, Academy of Orthopedics, Guangdong Province, The Third Affiliated Hospital, Southern Medical University, Guangzhou, 510665, China.
Department of Orthopedics & Microsurgery, Guangdong No. 2 Provincial People's Hospital, Guangzhou, 510317, China.
J Orthop Res. 2015 Sep;33(9):1356-63. doi: 10.1002/jor.22825. Epub 2015 Jun 26.
The roles of different immune cell populations and cytokines in bone metabolism have been extensively investigated. However, the influence of whole immune organ removal on osteopathology remains unknown. In the current study, we investigated the effects of splenectomy on bone metabolism and microarchitecture in rats with or without concurrent ovariectomy. Ovariectomized (OVX) rats were used as osteoporosis model. Sixty 12-week-old female rats were randomized into 4 groups (n = 15): sham, splenectomized (SP), ovariectomized, as well as ovariectomized and splenectomized (OVX + SP). Bone microarchitecture was assessed by micro CT analysis at 4 week and 12 week post-operation, respectively. Bone pathology and metabolism were evaluated via immunohistochemical staining. The serum levels of alkaline phosphatase (ALP), tumor necrosis factor-alpha (TNF-α), tartrate-resistant acid phosphatase 5b (Tracp5b), and C-terminal telopeptide (CTx) were analyzed at 4 and 12 weeks post-operation. Removal of the spleen led to alterations in the homeostasis of bone metabolism and increased bone formation in rats. In this study, our findings indicate that the spleen is involved in skeletal metabolism.
不同免疫细胞群体和细胞因子在骨代谢中的作用已得到广泛研究。然而，整个免疫器官切除对骨病理学的影响仍不清楚。在本研究中，我们研究了脾切除术对有或无同时进行卵巢切除术的大鼠骨代谢和微观结构的影响。去卵巢（OVX）大鼠用作骨质疏松模型。将60只12周龄雌性大鼠随机分为4组（n = 15）：假手术组、脾切除组（SP）、去卵巢组以及去卵巢并脾切除组（OVX + SP）。分别在术后4周和12周通过显微CT分析评估骨微观结构。通过免疫组织化学染色评估骨病理学和代谢情况。在术后4周和12周分析血清碱性磷酸酶（ALP）、肿瘤坏死因子-α（TNF-α）、抗酒石酸酸性磷酸酶5b（Tracp5b）和C-末端肽（CTx）的水平。脾切除导致大鼠骨代谢稳态改变并增加骨形成。在本研究中，我们的发现表明脾脏参与骨骼代谢。
