光散射变化与Cdc42调节的肌动蛋白状态的关系。
Relationship of light scatter change and Cdc42-regulated actin status.
作者信息
Hong Lin, Chavez Stephanie, Smagley Yelena, Chigaev Alexandre, Sklar Larry A
机构信息
Department of Pathology, The University of New Mexico, Albuquerque, New Mexico, 87131.
Department of Pathology, Center for Molecular Discovery, The University of New Mexico, Albuquerque, New Mexico, 87131.
出版信息
Cytometry B Clin Cytom. 2016 Nov;90(6):499-505. doi: 10.1002/cyto.b.21223. Epub 2015 Jul 3.
BACKGROUND
Cdc42 GTPase has important roles in regulating intracellular actin reorganization. The current methods to monitor actin changes are typically complex and point by point.
METHODS
The effects of Cdc42 inhibitors on the side scatter changes were tested in a newly developed continuous assay using the flow cytometer. Staining with fluorescently labeled phalloidin was used for comparison.
RESULTS
Cdc42-specific inhibitors caused dose-dependent changes of both the right-angle side scatter and the phalloidin-stained actin.
CONCLUSIONS
The right-angle light scatter change can be used as a method to circumvent phalloidin staining and be an early convenient step in screening Cdc42 inhibitors. © 2015 International Clinical Cytometry Society.
背景
Cdc42 GTP酶在调节细胞内肌动蛋白重组中起重要作用。目前监测肌动蛋白变化的方法通常很复杂且逐点进行。
方法
使用流式细胞仪在新开发的连续检测中测试Cdc42抑制剂对侧向散射变化的影响。用荧光标记的鬼笔环肽染色用于比较。
结果
Cdc42特异性抑制剂引起直角侧向散射和鬼笔环肽染色的肌动蛋白的剂量依赖性变化。
结论
直角光散射变化可作为一种规避鬼笔环肽染色的方法，并且是筛选Cdc42抑制剂的早期便捷步骤。©2015国际临床细胞计量学会。
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