氧化锌纳米颗粒对硕大利什曼原虫（MRHO/IR/75/ER）前鞭毛体和无鞭毛体形式细胞毒性作用的体外研究
In Vitro Study on Cytotoxic Effects of ZnO Nanoparticles on Promastigote and Amastigote Forms of Leishmania major (MRHO/IR/75/ER).
作者信息
Delavari Mahdi, Dalimi Abdolhossein, Ghaffarifar Fatemeh, Sadraei Javid
机构信息
Dept. of Parasitology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.
出版信息
Iran J Parasitol. 2014 Mar;9(1):6-13.
BACKGROUND
Although pentavalent antimony compounds are used as antileishmanial drugs but they are associated with limitations and several adverse complications. Therefore, always effort to find a new and effective treatment is desired. In this study, the effect of ZnO nanoparticles with mean particle size of 20 nanometers (nm) on Leishmania major promastigotes and amastigotes was evaluated.
METHODS
Viability percentage of promastigotes after adding different concentrations of ZnO nanoparticles (30, 60, 90 and 120 μg/ml) to the parasite culture was evaluated by MTT assay. In the flow cytometry study, Annexin V-FITC Apoptosis detection Kit was used to study the induced apoptosis and necrotic effects.
RESULT
IC50 after 24 hours of incubation was 37.8 μg/ml. ZnO nanoparticles exert cytotoxic effects on promastigotes of L. major through the induction of apoptosis. A concentration of 120 μg/ml of ZnO nanoparticles induced 93.76% apoptosis in L. major after 72 hours.
CONCLUSION
ZnO NPs can induce apoptosis in L. major by dose and time-depended manner in vitro condition.
背景
尽管五价锑化合物被用作抗利什曼原虫药物，但它们存在局限性并伴有多种不良并发症。因此，一直需要努力寻找新的有效治疗方法。在本研究中，评估了平均粒径为20纳米（nm）的氧化锌纳米颗粒对硕大利什曼原虫前鞭毛体和无鞭毛体的影响。
方法
通过MTT法评估向寄生虫培养物中添加不同浓度的氧化锌纳米颗粒（30、60、90和120μg/ml）后前鞭毛体的活力百分比。在流式细胞术研究中，使用膜联蛋白V-FITC凋亡检测试剂盒研究诱导的凋亡和坏死效应。
结果
孵育24小时后的半数抑制浓度（IC50）为37.8μg/ml。氧化锌纳米颗粒通过诱导凋亡对硕大利什曼原虫前鞭毛体产生细胞毒性作用。120μg/ml的氧化锌纳米颗粒在72小时后诱导硕大利什曼原虫93.76%的凋亡。
结论
在体外条件下，氧化锌纳米颗粒可通过剂量和时间依赖性方式诱导硕大利什曼原虫凋亡。
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