Asia Glycomics Reference Site , Daejeon 305-764, Korea.
Anal Chem. 2015 Mar 3;87(5):2869-77. doi: 10.1021/ac504339t. Epub 2015 Feb 13.
Tissue glyco-capture (TGC), a highly sensitive MS-compatible method for extraction of glycans from tissue, was combined with structure-specific nano-LC/MS for sensitive and detailed profiling of the mouse brain glycome. Hundreds of glycan structures were directly detected by accurate mass MS and structurally elucidated by MS/MS, revealing the presence of novel glycan motifs such as antennary fucosylation, sulfation, and glucuronidation that are potentially associated with cellular signaling and adhesion. Microgram-level sensitivity enabled glycomic analysis of specific regions of the brain, as demonstrated on not only brain sections (with a one-dimensional spatial resolution of 20 μm) but also isolated brain structures (e.g., the hippocampus). Reproducibility was extraordinarily high (R > 0.98) for both method and instrumental replicates. The pairing of TGC with structure-specific nano-LC/MS was found to be an exceptionally powerful platform for qualitative and quantitative exploration of the brain glycome.
组织糖捕获（TGC）是一种高度灵敏的 MS 兼容方法，可从组织中提取聚糖，与结构特异性纳升液相色谱/质谱联用，用于敏感和详细分析小鼠大脑糖组。通过精确质量 MS 直接检测到数百种聚糖结构，并通过 MS/MS 进行结构解析，揭示了潜在与细胞信号和黏附相关的新型聚糖基序，如天线岩藻糖基化、硫酸化和葡糖醛酸化。微克级的灵敏度使大脑特定区域的糖组学分析成为可能，不仅在脑切片上（具有 20 μm 的一维空间分辨率），而且在分离的脑结构上（例如海马体）也可以进行分析。方法和仪器重复的重现性非常高（R > 0.98）。TGC 与结构特异性纳升液相色谱/质谱联用被发现是一种非常强大的平台，可用于定性和定量探索大脑糖组。
