Hua Serenus, Lebrilla Carlito, An Hyun Joo
Department of Chemistry, University of California, Davis, CA 95616, USA.
Bioanalysis. 2011 Nov;3(22):2573-85. doi: 10.4155/bio.11.263.
The glycome, that is, the glycan components of a biological source, has been widely reported to change with disease states. However, mining the glycome for biomarkers is complicated by glycan structural heterogeneity. Nanoflow LC, or nano-LC, significantly addresses the problem by providing a highly sensitive and quantitative method of separating and profiling glycans. This review summarizes recent advances in analytical technology and methodology that enhance and augment the advantages offered by nano-LC. (e.g., reversed phase, hydrophilic interaction and porous graphitized carbon chromatography, as well as associated derivatization strategies), detectors (e.g., fluorescence and MS), and technology platforms (particularly chip-based nano-LC) are examined in detail, along with their application to biomarker discovery. Particular emphasis is placed on methods and technologies that allow structure-specific glycan profiling.
糖组，即生物源的聚糖成分，已被广泛报道会随疾病状态而变化。然而，由于聚糖结构的异质性，从糖组中挖掘生物标志物变得很复杂。纳流液相色谱（nanoflow LC，或nano-LC）通过提供一种高度灵敏且定量的聚糖分离和分析方法，显著解决了这一问题。本综述总结了分析技术和方法的最新进展，这些进展增强并扩大了nano-LC所具有的优势。（例如反相、亲水相互作用和多孔石墨化碳色谱法，以及相关的衍生化策略）、检测器（例如荧光和质谱）和技术平台（特别是基于芯片的nano-LC）均得到了详细研究，并探讨了它们在生物标志物发现中的应用。特别强调了能够进行结构特异性聚糖分析的方法和技术。
