人类CD2基因3'侧翼序列在转基因小鼠中赋予高水平、T细胞特异性、位置独立的基因表达。

Human CD2 3'-flanking sequences confer high-level, T cell-specific, position-independent gene expression in transgenic mice.

作者信息

Greaves D R, Wilson F D, Lang G, Kioussis D

机构信息

Laboratory of Gene Structure and Expression, National Institute for Medical Research, London, England.

出版信息

Cell. 1989 Mar 24;56(6):979-86. doi: 10.1016/0092-8674(89)90631-4.

DOI:10.1016/0092-8674(89)90631-4

PMID:2564317

Abstract

We have localized a set of T cell-specific DNAase I hypersensitive sites in the 3'-flanking region of the human CD2 gene. A 5.5 kb BamHI-XbaI fragment containing these DNAase I hypersensitive sites conferred efficient, copy number-dependent, T cell-specific expression of a linked human CD2 minigene, independent of the position of integration in the transgenic mouse genome. When linked to the mouse Thy-1.1 gene or the human beta-globin gene, this fragment conferred the same T cell-specific expression, independent of its orientation. These results suggest that this flanking region is both necessary and sufficient for full tissue-specific activation of homologous and heterologous genes in transgenic mice.

摘要

我们已经在人类CD2基因的3'侧翼区域定位了一组T细胞特异性脱氧核糖核酸酶I超敏位点。一个包含这些脱氧核糖核酸酶I超敏位点的5.5 kb BamHI - XbaI片段赋予了与之相连的人类CD2小基因高效的、拷贝数依赖的、T细胞特异性表达，且与转基因小鼠基因组中的整合位置无关。当与小鼠Thy - 1.1基因或人类β - 珠蛋白基因相连时，该片段赋予相同的T细胞特异性表达，且与它的方向无关。这些结果表明，该侧翼区域对于转基因小鼠中同源和异源基因的完全组织特异性激活既是必要的也是充分的。