Lang G, Wotton D, Owen M J, Sewell W A, Brown M H, Mason D Y, Crumpton M J, Kioussis D
Laboratory of Gene Structure and Expression, National Institute for Medical Research, Mill Hill, London, UK.
EMBO J. 1988 Jun;7(6):1675-82. doi: 10.1002/j.1460-2075.1988.tb02995.x.
We report the genomic organization of the human CD2 gene and its expression in transgenic mice. A 28.5 kb segment of DNA consisting of 4.5 kb 5' flanking sequences, 15 kb containing the gene's five exons and 9 kb of 3' flanking sequences can direct the expression of the CD2 gene only on thymocytes, circulating T cells and megakaryocytes of the transgenic mice. The expression of each copy of the human CD2 transgene appears to be as high as the endogenous mouse CD2 gene and as high as the expression on the surface of human T lymphocytes, independent of the site of integration and dependent on the copy number of genes that have integrated.
我们报告了人类CD2基因的基因组结构及其在转基因小鼠中的表达。一段28.5 kb的DNA片段，由4.5 kb的5'侧翼序列、包含该基因五个外显子的15 kb以及9 kb的3'侧翼序列组成，可仅在转基因小鼠的胸腺细胞、循环T细胞和巨核细胞中指导CD2基因的表达。人类CD2转基因的每个拷贝的表达似乎与内源性小鼠CD2基因一样高，并且与人类T淋巴细胞表面的表达一样高，与整合位点无关，且取决于整合的基因拷贝数。
