Johari-Ahar M, Rashidi M R, Barar J, Aghaie M, Mohammadnejad D, Ramazani A, Karami P, Coukos G, Omidi Y
Research Centre for Pharmaceutical Nanotechnology, Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran.
Nanoscale. 2015 Feb 28;7(8):3768-79. doi: 10.1039/c4nr06687a.
Effective treatment of ovarian cancer depends upon the early detection of the malignancy. Here, we report on the development of a new nanostructured immunosensor for early detection of cancer antigen 125 (CA-125). A gold electrode was modified with mercaptopropionic acid (MPA), and then consecutively conjugated with silica coated gold nanoparticles (AuNP@SiO2), CdSe quantum dots (QDs) and anti-CA-125 monoclonal antibody (mAb). The engineered MPA|AuNP@SiO2|QD|mAb immunosensor was characterised using transmission electron microscopy (TEM), atomic force microscopy (AFM), cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). Successive conjugation of AuNP@SiO2, CdSe QD and anti-CA-125 mAb onto the gold electrode resulted in sensitive detection of CA-125 with a limit of detection (LOD) of 0.0016 U mL(-1) and a linear detection range (LDR) of 0-0.1 U mL(-1). Based on the high sensitivity and specificity of the immunosensor, we propose this highly stable and reproducible biosensor for the early detection of CA-125.
卵巢癌的有效治疗取决于恶性肿瘤的早期检测。在此，我们报告一种用于早期检测癌抗原125（CA-125）的新型纳米结构免疫传感器的研制。用巯基丙酸（MPA）修饰金电极，然后依次与二氧化硅包覆的金纳米颗粒（AuNP@SiO2）、CdSe量子点（QDs）和抗CA-125单克隆抗体（mAb）共轭。采用透射电子显微镜（TEM）、原子力显微镜（AFM）、循环伏安法（CV）和电化学阻抗谱（EIS）对工程化的MPA|AuNP@SiO2|QD|mAb免疫传感器进行了表征。将AuNP@SiO2、CdSe QD和抗CA-125 mAb依次共轭到金电极上，实现了对CA-125的灵敏检测，检测限（LOD）为0.0016 U mL(-1)，线性检测范围（LDR）为0-0.1 U mL(-1)。基于该免疫传感器的高灵敏度和特异性，我们提出将这种高度稳定且可重复的生物传感器用于CA-125的早期检测。
