Graduate Institute of Applied Science and Technology, National Taiwan University of Science and Technology, Taipei 106, Taiwan.
R&D Center for Membrane Technology, Chung Yuan Christian University, Taoyuan 320, Taiwan.
Sensors (Basel). 2023 Jan 18;23(3):1131. doi: 10.3390/s23031131.
Developing label-free immunosensors to detect ovarian cancer (OC) by cancer antigen (CA125) is essential to improving diagnosis and protecting women from life-threatening diseases. Four types of carbon nanomaterials, such as multi-wall carbon nanotubes (MWCNTs), vapor-grown carbon fiber (VGCFs), graphite KS4, and carbon black super P (SP), have been treated with acids to prepare a carbon nanomaterial/gold (Au) nanocomposite. The AuNPs@carbon nanocomposite was electrochemically deposited on a glassy carbon electrode (GCE) to serve as a substrate to fabricate a label-free immunosensor for the detection of CA125. Among the four AuNPs@carbon composite, the AuNPs@MWCNTs-based sensor exhibited a high sensitivity of 0.001 µg/mL for the biomarker CA125 through the square wave voltammetry (SWV) technique. The high conductivity and surface area of MWCNTs supported the immobilization of AuNPs. Moreover, the carboxylic (COO-) functional groups in MWCNT improved to a higher quantity after the acid treatment, which served as an excellent support for the fabrication of electrochemical biosensors. The present method aims to explore an environmentally friendly synthesis of a layer-by-layer (LBL) assembly of AuNPs@carbon nanomaterials electrochemical immunoassay to CA125 in a clinical diagnosis at a low cost and proved feasible for point-of-care diagnosis.
开发用于通过肿瘤抗原（CA125）检测卵巢癌（OC）的无标记免疫传感器对于改善诊断和保护女性免受危及生命的疾病至关重要。已经用酸处理了四种类型的碳纳米材料，例如多壁碳纳米管（MWCNTs）、气相生长碳纤维（VGCFs）、石墨 KS4 和碳黑超级 P（SP），以制备碳纳米材料/金（Au）纳米复合材料。将 AuNPs@碳纳米复合材料电化学沉积在玻碳电极（GCE）上，用作制备用于检测 CA125 的无标记免疫传感器的基底。在这四种 AuNPs@碳复合材料中，基于 AuNPs@MWCNTs 的传感器通过方波伏安法（SWV）技术表现出对生物标志物 CA125 的高灵敏度为 0.001 µg/mL。MWCNTs 的高导电性和表面积支持 AuNPs 的固定化。此外，酸处理后 MWCNT 中的羧酸（COO-）官能团增加到更高的数量，这为电化学生物传感器的制备提供了极好的支持。本方法旨在探索一种环保的方法，通过层层（LBL）组装将 AuNPs@碳纳米材料电化学免疫分析用于临床诊断中的 CA125，成本低廉，并证明适用于即时诊断。
