基于聚合物的铽配合物作为癌抗原125检测的荧光探针：一种用于卵巢癌早期诊断的有前景的工具。
Polymer-Based Terbium Complex as a Fluorescent Probe for Cancer Antigen 125 Detection: A Promising Tool for Early Diagnosis of Ovarian Cancer.
作者信息
Mohamed Magda M, Gamal Hisham, El-Didamony Akram, Youssef Ahmed O, Elshahat Esraa, Mohamed Ekram H, Attia Mohamed S
机构信息
Chemistry Department, Faculty of Science, Ain Shams University, Abbassia, Cairo 11566, Egypt.
Aeromedical Council Laboratories-Ministry of Civil Aviation, Cairo 3753450, Egypt.
出版信息
ACS Omega. 2024 Jun 1;9(23):24916-24924. doi: 10.1021/acsomega.4c01814. eCollection 2024 Jun 11.
A novel photoprobe, Tb-acetylacetone (Tb-ACAC) doped within a modified epoxy cellulose polymer immobilized with CA-125 monoclonal antibody, offers an accurate and highly selective method for early ovarian cancer (OC) diagnosis by detecting cancer antigen 125 (CA-125) in serum samples. This approach leverages quenching of the Tb-ACAC luminescence upon binding to CA-125. Characterization of the photoprobe film through UV-vis and fluorescence measurements confirmed the presence of Tb-ACAC within the polymer matrix. In aqueous solution (pH 6.8, λ = 365 nm), the characteristic emission band of Tb-ACAC at λ = 546.2 nm exhibited significant quenching upon CA-125 binding. This quenching effect enabled the sensitive and specific detection of CA-125 in diverse serum samples from OC patients, demonstrating the applicability, simplicity, and effectiveness of this novel approach.
一种新型光探针，即掺杂在固定有CA - 125单克隆抗体的改性环氧纤维素聚合物中的铽 - 乙酰丙酮（Tb - ACAC），通过检测血清样本中的癌抗原125（CA - 125），为早期卵巢癌（OC）诊断提供了一种准确且高度选择性的方法。该方法利用了Tb - ACAC与CA - 125结合时其发光的猝灭现象。通过紫外 - 可见光谱和荧光测量对光探针薄膜进行表征，证实了聚合物基质中存在Tb - ACAC。在水溶液（pH 6.8，λ = 365 nm）中，当与CA - 125结合时，Tb - ACAC在λ = 546.2 nm处的特征发射带表现出显著的猝灭。这种猝灭效应能够灵敏且特异地检测来自OC患者的各种血清样本中的CA - 125，证明了这种新方法的适用性、简便性和有效性。
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