Antioxidants, Free Radicals, Functional Foods and Toxicology Research Laboratory, Department of Biological Sciences, Al-Hikmah University, Ilorin, Nigeria.
J Ethnopharmacol. 2015 Apr 22;164:273-82. doi: 10.1016/j.jep.2015.01.026. Epub 2015 Jan 30.
Vitex doniana fruits are locally used in Nigeria as a remedy in the treatment of jaundice and liver related disease. The effect of methanolic extract of Vitex doniana fruits on acetaminophen induced protein oxidation, lipid peroxidation and DNA fragmentation was investigated in mice.
Antioxidant activity of the extract (0.2-1.0mg/mL) was investigated in vitro using 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical, superoxide ion, hydrogen peroxide, hydroxyl radical and ferric ion reducing system. Vitex doniana extract at 1.0mg/mL scavenged DPPH, superoxide ion, hydrogen peroxide, and hydroxyl radical by 86%, 78%, 80% and 72% respectively, it also reduced ferric ion significantly. Hepatoprotective effect of Vitex doniana fruits was monitored in acetaminophen-induced hepatotoxicity in mice.
Acetaminophen-mediated alterations in serum alkaline phosphatase, alanine aminotransferase, aspartate aminotransferase, albumin and total bilirubin levels in mice were significantly (P<0.05) attenuated by the extract. Similarly, acetaminophen-mediated decrease in activities of superoxide dismutase, catalase, glutathione peroxidase, glutathione reductase and glucose 6-phosphate dehydrogenase was significantly (P<0.05) attenuated in the liver of mice. Increased levels of conjugated dienes, lipid hydroperoxides, malondialdehyde, protein carbonyl and fragmented DNA were significantly (P<0.05) lowered by methanolic extract of Vitex doniana fruits.
Overall, the results of this study show that Vitex doniana fruits possess antioxidant properties and halted acetaminophen-mediated oxidative rout on cellular proteins, lipids and DNA, made possible by β-sitosterol, platycodin D, apigenin, saikosaponin, chrysin and ellagitanin in the extract.
多花山竹子的果实，在尼日利亚当地被用作治疗黄疸和肝脏相关疾病的药物。本研究旨在探讨多花山竹子果实的甲醇提取物对醋氨酚诱导的蛋白质氧化、脂质过氧化和 DNA 片段化的影响。
采用 2,2-二苯基-1-苦肼基（DPPH）自由基、超氧阴离子、过氧化氢、羟自由基和铁离子还原系统，在体外研究提取物（0.2-1.0mg/mL）的抗氧化活性。多花山竹子提取物在 1.0mg/mL 时，对 DPPH、超氧阴离子、过氧化氢和羟自由基的清除率分别为 86%、78%、80%和 72%，对铁离子的还原作用也很显著。监测多花山竹子果实对醋氨酚诱导的小鼠肝毒性的肝保护作用。
醋氨酚介导的小鼠血清碱性磷酸酶、丙氨酸氨基转移酶、天冬氨酸氨基转移酶、白蛋白和总胆红素水平的变化，均被提取物显著（P<0.05）减弱。同样，醋氨酚介导的超氧化物歧化酶、过氧化氢酶、谷胱甘肽过氧化物酶、谷胱甘肽还原酶和葡萄糖 6-磷酸脱氢酶活性的降低，在小鼠肝脏中也显著（P<0.05）减弱。多花山竹子果实的甲醇提取物可显著（P<0.05）降低共轭二烯、脂质过氧化物、丙二醛、蛋白质羰基和断裂 DNA 的水平。
总的来说，本研究结果表明，多花山竹子果实具有抗氧化特性，并通过提取物中的β-谷甾醇、远志皂苷 D、芹菜素、柴胡皂苷、白杨素和鞣花单宁，阻止了醋氨酚介导的细胞蛋白质、脂质和 DNA 的氧化途径。
