Department of Nutrition and Food Hygiene, School of Public Health, Peking University, Beijing 100191, China.
Nutrients. 2021 Jun 17;13(6):2078. doi: 10.3390/nu13062078.
Peptic ulcer has a serious impact on people's health around the world, and traditional medicines can cause adverse reactions. This study investigated the protective effects of tilapia collagen oligopeptides (TCOPs) on gastroduodenal injury. Seventy-two specific pathogen-free (SPF) male Sprague Dawley (SD) rats were randomly divided into six groups according to body weight: normal control group, ethanol group, whey protein group (500 mg/kg BW), and three TCOPs dose groups (250, 500, 1000 mg/kg BW). After intragastric administration for 30 days, the acute gastroduodenal injury was induced by anhydrous ethanol (5 mL/kg, intragastrically) in all groups except the normal control group. Biomarkers in gastric and duodenal tissue and serum were measured. Furthermore, western blot was used to detect the expression of apoptosis-related proteins. The results showed that the administration with TCOPs significantly reduced gastric and duodenal ulcer index, increased gastric juice pH, superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px) activities, along with the reduction of malondialdehyde (MDA) contents. TCOPs decreased tumor Necrosis Factor-α (TNF-α), interleukin-1β (IL-1β), and myeloperoxidase (MPO) levels, while interleukin- 10 (IL-10) levels were increased. Furthermore, pepsinogens 1 (PG1), pepsinogens 2 (PG2), gastrin (GAS), and the pepsinogen ratio (PGR) were decreased, the prostaglandin E2 (PGE2) and NO contents were increased after TCOPs intervention. Moreover, TCOPs up-regulated the expression of Bcl-2 and inhibited the expression of Bax and Caspase-3. In conclusion, TCOPs have protective effects on ethanol-induced gastroduodenal injury through gastrointestinal mucosal microcirculation promotion, antioxidation, anti-inflammation, and anti-apoptosis mechanisms.
消化性溃疡对全世界人民的健康造成了严重影响，而传统药物可能会引起不良反应。本研究探讨了罗非鱼胶原蛋白寡肽（TCOPs）对胃十二指肠损伤的保护作用。72 只特定病原体无菌（SPF）雄性 Sprague Dawley（SD）大鼠按体重随机分为 6 组：正常对照组、乙醇组、乳清蛋白组（500mg/kgBW）和 3 个 TCOPs 剂量组（250、500、1000mg/kgBW）。除正常对照组外，所有组均经胃内给予无水乙醇（5mL/kg）诱导急性胃十二指肠损伤，测定胃和十二指肠组织及血清中的生物标志物，并采用 Western blot 检测凋亡相关蛋白的表达。结果表明，TCOPs 给药可显著降低胃和十二指肠溃疡指数，增加胃液 pH 值，提高超氧化物歧化酶（SOD）、过氧化氢酶（CAT）和谷胱甘肽过氧化物酶（GSH-Px）活性，降低丙二醛（MDA）含量。TCOPs 降低肿瘤坏死因子-α（TNF-α）、白细胞介素-1β（IL-1β）和髓过氧化物酶（MPO）水平，增加白细胞介素-10（IL-10）水平。此外，TCOPs 干预后胃蛋白酶原 1（PG1）、胃蛋白酶原 2（PG2）、胃泌素（GAS）和胃蛋白酶原比值（PGR）降低，前列腺素 E2（PGE2）和一氧化氮（NO）含量增加。此外，TCOPs 上调 Bcl-2 的表达，抑制 Bax 和 Caspase-3 的表达。综上所述，TCOPs 通过促进胃肠道黏膜微循环、抗氧化、抗炎和抗凋亡机制对乙醇诱导的胃十二指肠损伤具有保护作用。
