Zou Junhui, Wang Wenbin, Pan Yung-Wei, Lu Song, Xia Zhengui
Toxicology Program, Department of Environmental and Occupational Health Sciences, University of Washington, Seattle, Washington.
Graduate Program, Molecular and Cellular Biology, University of Washington, Seattle, Washington.
Curr Protoc Toxicol. 2015 Feb 2;63:11.18.1-11.18.21. doi: 10.1002/0471140856.tx1118s63.
Mice rely on the sense of olfaction to detect food sources, recognize social and mating partners, and avoid predators. Many behaviors of mice, including learning and memory, social interaction, fear, and anxiety are closely associated with their function of olfaction, and behavior tasks designed to evaluate those brain functions may use odors as cues. Accurate assessment of olfaction is not only essential for the study of olfactory system but also critical for proper interpretation of various mouse behaviors, especially learning and memory, emotionality and affect, and sociality. Here we describe a series of behavior experiments that offer multidimensional and quantitative assessments for mouse olfactory function, including olfactory habituation, discrimination, odor preference, odor detection sensitivity, and olfactory memory, with respect to both social and nonsocial odors.
小鼠依靠嗅觉来探测食物来源、识别社交和交配伙伴以及躲避捕食者。小鼠的许多行为，包括学习和记忆、社交互动、恐惧和焦虑，都与其嗅觉功能密切相关，而旨在评估这些脑功能的行为任务可能会使用气味作为线索。准确评估嗅觉不仅对于嗅觉系统的研究至关重要，而且对于正确解释各种小鼠行为，尤其是学习和记忆、情绪和情感以及社交性也至关重要。在此，我们描述了一系列行为实验，这些实验针对社交和非社交气味，对小鼠嗅觉功能提供了多维度和定量评估，包括嗅觉习惯化、辨别、气味偏好、气味检测灵敏度和嗅觉记忆。
