Srisawasdi Pornpen, Vanavanan Somlak, Rochanawutanon Mana, Kruthkul Khanat, Kotani Kazuhiko, Kroll Martin H
Department of Pathology, Faculty of Medicine, Ramathibodi Hospital, Mahidol University, Bangkok 10400, Thailand.
Department of Pathology, Faculty of Medicine, Ramathibodi Hospital, Mahidol University, Bangkok 10400, Thailand.
Clin Biochem. 2015 May;48(7-8):495-502. doi: 10.1016/j.clinbiochem.2015.01.011. Epub 2015 Jan 31.
Heterogeneous particles of intermediate-density lipoprotein (IDL) and low-density lipoprotein (LDL) vary in atherogenesis. We investigated the association between the metabolic syndrome (MetS) score and lipoprotein subclasses.
A total of 260 outpatients were scored into six groups, based on their number of MetS components. Lipoprotein subclass determined by polyacrylamide tube gel electrophoresis separates IDL particles into three midbands (MID-A to C) and LDL into larger-buoyant (LDL1 and LDL2) and small-dense LDL (LDL3 to LDL6).
Mean concentrations of VLDL, MIDC, LDL2, and LDL3 to LDL6 positively correlated with increasing MetS score, but those of MIDA, LDL1 and HDL-C inversely correlated. LDL2 and LDL3 to LDL6 increased while MIDA and LDL1 decreased with increasing visceral fat, HOMA-IR, and triglycerides, with a reverse pattern for HDL-C. MIDB and MIDC were unchanged. By logistic regression, LDL1 and LDL3 to LDL6 significantly associates with the MetS score (odds ratio=0.957 and 1.077, respectively). The ratio of (LDL3 to LDL6)/LDL1 in the presence of HDL-C, showed the strongest association with MetS.
Respective subpopulations of IDL and LDL particles can vary in their ability to identify MetS. Because of the most strongly associated with MetS, (LDL3 to LDL6)/LDL1 ratio is proposed as an excellent marker for evaluating lipid metabolic status in patient with MetS.
中等密度脂蛋白（IDL）和低密度脂蛋白（LDL）的异质性颗粒在动脉粥样硬化发生过程中存在差异。我们研究了代谢综合征（MetS）评分与脂蛋白亚类之间的关联。
根据260名门诊患者的MetS组分数量将其分为六组。通过聚丙烯酰胺管凝胶电泳测定的脂蛋白亚类将IDL颗粒分为三个中带（MID-A至C），将LDL分为较大浮力的（LDL1和LDL2）和小而密的LDL（LDL3至LDL6）。
VLDL、MIDC、LDL2以及LDL3至LDL6的平均浓度与MetS评分升高呈正相关，而MIDA、LDL1和HDL-C的平均浓度与之呈负相关。随着内脏脂肪、HOMA-IR和甘油三酯升高，LDL2以及LDL3至LDL6升高，而MIDA和LDL1降低，HDL-C则呈现相反模式。MIDB和MIDC保持不变。通过逻辑回归分析，LDL1以及LDL3至LDL6与MetS评分显著相关（优势比分别为0.957和1.077）。在存在HDL-C的情况下，（LDL3至LDL6）/LDL1的比值与MetS的关联最为强烈。
IDL和LDL颗粒的各个亚群在识别MetS的能力方面可能存在差异。由于（LDL3至LDL6）/LDL1比值与MetS的关联最为强烈，因此建议将其作为评估MetS患者脂质代谢状态的优秀标志物。
