Tian Lifeng, Kazmierkiewicz Krista L, Bowman Anita S, Li Mingyao, Curcio Christine A, Stambolian Dwight E
Center for Applied Genomics, The Children's Hospital of Philadelphia, Philadelphia, Pa 19104, USA.
Department of Ophthalmology, University of Pennsylvania, Philadelphia, Pa 19104, USA.
Genomics. 2015 May;105(5-6):253-64. doi: 10.1016/j.ygeno.2015.01.008. Epub 2015 Jan 31.
The retina and its adjacent supporting tissues - retinal pigmented epithelium (RPE) and choroid - are critical structures in human eyes required for normal visual perception. Abnormal changes in these layers have been implicated in diseases such as age-related macular degeneration and glaucoma. With the advent of high-throughput methods, such as serial analysis of gene expression, cDNA microarray, and RNA sequencing, there is unprecedented opportunity to facilitate our understanding of the normal retina, RPE, and choroid. This information can be used to identify dysfunction in age-related macular degeneration and glaucoma. In this review, we describe the current status in our understanding of these transcriptomes through the use of high-throughput techniques.
视网膜及其相邻的支持组织——视网膜色素上皮（RPE）和脉络膜——是人类眼睛中正常视觉感知所需的关键结构。这些层的异常变化与年龄相关性黄斑变性和青光眼等疾病有关。随着高通量方法的出现，如基因表达序列分析、cDNA微阵列和RNA测序，我们有前所未有的机会来促进对正常视网膜、RPE和脉络膜的理解。这些信息可用于识别年龄相关性黄斑变性和青光眼中的功能障碍。在本综述中，我们描述了通过使用高通量技术对这些转录组的当前理解状况。
