Department of Ophthalmology, Konkuk University School of Medicine, Seoul, Republic of Korea.
Department of Ophthalmology, Konkuk University Medical Center, Seoul, Republic of Korea.
Commun Biol. 2022 Jul 20;5(1):722. doi: 10.1038/s42003-022-03676-3.
Cellular senescence of the retinal pigment epithelium (RPE) is thought to play an important role in vision-threatening retinal degenerative diseases, such as age-related macular degeneration (AMD). However, the single-cell RNA profiles of control RPE tissue and RPE tissue exhibiting cellular senescence are not well known. We have analyzed the single-cell transcriptomes of control mice and mice with low-dose doxorubicin (Dox)-induced RPE senescence (Dox-RPE). Our results have identified 4 main subpopulations in the control RPE that exhibit heterogeneous biological activities and play roles in ATP synthesis, cell mobility/differentiation, mRNA processing, and catalytic activity. In Dox-RPE mice, cellular senescence mainly occurs in the specific cluster, which has been characterized by catalytic activity in the control RPE. Furthermore, in the Dox-RPE mice, 6 genes that have not previously been associated with senescence also show altered expression in 4 clusters. Our results might serve as a useful reference for the study of control and senescent RPE.
视网膜色素上皮 (RPE) 的细胞衰老被认为在威胁视力的视网膜退行性疾病中起重要作用，例如年龄相关性黄斑变性 (AMD)。然而，表现出细胞衰老的对照 RPE 组织和 RPE 组织的单细胞 RNA 图谱尚不清楚。我们已经分析了对照小鼠和接受低剂量阿霉素 (Dox) 诱导的 RPE 衰老 (Dox-RPE) 的小鼠的单细胞转录组。我们的结果在对照 RPE 中确定了 4 个主要亚群，它们表现出不同的生物活性，在 ATP 合成、细胞迁移/分化、mRNA 处理和催化活性中发挥作用。在 Dox-RPE 小鼠中，细胞衰老主要发生在对照 RPE 中具有催化活性的特定簇中。此外，在 Dox-RPE 小鼠中，先前与衰老无关的 6 个基因在 4 个簇中也表现出改变的表达。我们的结果可能为对照和衰老的 RPE 研究提供有用的参考。
