Rogers Benjamin A, Wu Zhen, Wei Bingchuan, Zhang Ximo, Cao Xiang, Alabi Oyeleye, Wirth Mary J
Department of Chemistry, Purdue University , 560 Oval Drive, West Lafayette, Indiana 47907, United States.
Anal Chem. 2015 Mar 3;87(5):2520-6. doi: 10.1021/ac504683d. Epub 2015 Feb 17.
Smaller particles have progressively led to higher efficiency in liquid chromatography, particularly for proteins, due to smaller diffusion distances. Particle diameter has recently entered the submicrometer region, with the back-pressure requirements alleviated by slip flow.
由于扩散距离更小,较小的颗粒逐渐提高了液相色谱的效率,尤其是对于蛋白质而言。颗粒直径最近已进入亚微米区域,通过滑流减轻了背压要求。