Hainsworth Steven, Hamblin John F, Vanniasinkam Thiru
a Beaconsfield Podiatry.
J Am Podiatr Med Assoc. 2015 Feb 3. doi: 10.7547/13-151.1.
Background: Podiatric physicians routinely use electric drills for the treatment of nail and skin conditions. The grinding process produces human nail and skin dust that is generally vacuumed into bags in the grinding unit. Many of the nails are thought to be mycotic, particularly because they are obtained from patients with symptoms of dermatophyte infections. Currently, there is limited information available on the detection of fungi from nail dust samples. Herein, we attempt to address this situation and outline some of the difficulties that pathology laboratories face in isolating and identifying dermatophytes from nail samples. Methods: Fifty nail dust bags from podiatric medical clinics across all of the states and territories of Australia were collected and analyzed. Samples from the bags were inoculated onto primary isolation media. Fungal colonies that grew were then inoculated onto potato dextrose agar for identification using standard morphological (macroscopic and microscopic) features. Results: One hundred fifty-one colonies of dermatophytes were identified from 43 of the 50 samples. In addition 471 nondermatophyte molds were isolated, along with some yeasts and bacteria. Conclusions: The most common dermatophytes isolated were from the Trichophyton mentagrophytes/interdigitale complexes. Trichophyton rubrum, Trichophyton tonsurans, Trichophyton soudanense, and Epidermophyton floccosum were also isolated. An unidentified group of dermatophytes was also present. The three most common genera of nondermatophyte molds were Aspergillus, Penicillium, and Scopulariopsis, all of which have been implicated in onychomycosis and more general disease. The presence of viable fungal pathogens in the dust could potentially pose a health problem to podiatric physicians.
足病医生在治疗指甲和皮肤疾病时经常使用电钻。打磨过程会产生人指甲和皮肤粉尘，这些粉尘通常会被吸进打磨装置中的袋子里。许多指甲被认为是霉菌感染的，特别是因为它们取自患有皮肤癣菌感染症状的患者。目前，关于从指甲粉尘样本中检测真菌的信息有限。在此，我们试图解决这一情况，并概述病理实验室在从指甲样本中分离和鉴定皮肤癣菌时面临的一些困难。方法：收集并分析了来自澳大利亚所有州和领地的足病诊所的50个指甲粉尘袋。将袋子里的样本接种到初次分离培养基上。然后将生长出的真菌菌落接种到马铃薯葡萄糖琼脂上，利用标准的形态学（宏观和微观）特征进行鉴定。结果：在50个样本中的43个样本中鉴定出151个皮肤癣菌菌落。此外，还分离出471个非皮肤癣菌霉菌，以及一些酵母菌和细菌。结论：分离出的最常见皮肤癣菌来自须癣毛癣菌/指间毛癣菌复合体。还分离出红色毛癣菌、断发毛癣菌、苏丹毛癣菌和絮状表皮癣菌。还存在一组未鉴定的皮肤癣菌。非皮肤癣菌霉菌最常见的三个属是曲霉属、青霉属和帚霉属，所有这些都与甲癣和更普遍的疾病有关。粉尘中存在有活力的真菌病原体可能会对足病医生构成健康问题。
